Abstract
Objective: To evaluate in vitro the effect of a red propolis ethanolic extract (RPE) in the prevention of growth of a cariogenic biofilm and its cytotoxic potential. Material and Methods: Minimum inhibitory and bactericidal concentrations (MIC and MBC) of RPE against Streptococcus mutans and Lactobacillus casei were determined. The cytotoxic potential of 0.4% RPE in oral fibroblasts was observed after 1, 3 and 5 min of contact. Cellulose membrane disks (13 mm, N=12) were used for biofilm formation (24 h) of S. mutans and L. casei , which were treated (1 min) with 0.4% RPE or 0.12% Chlorhexidine (CHX). The control group of biofilm formation was not submitted to any treatment. Serial dilutions were then made to evaluate microbial viability. Descriptive data analysis and, for microbial viability, Mann Whitney test were performed (p≤0.05). Results: RPE showed similar MIC and MBC (4.46 mg/mL) against S. mutans and, for L. casei , they were 8.92 mg/mL (MIC) and 17.85 mg/mL (MBC). CHX presented MIC and MBC 0.05). Regarding the total number of viable bacteria (Log 10 CFU/mL), the RPE (6.55) and CHX (6.87) presented similar efficacy (p>0.05). Conclusion: Red propolis extract showed antibacterial activity against the tested strains, exhibited acceptable cytotoxicity and reduced the colonization of S. mutans and L. casei in a biofilm membrane model.
Highlights
Dental caries results from surface demineralization caused by an organized biofilm exposed to fermentable carbohydrates from the diet [1]
Minimum Inhibitory Concentration (MIC) and Minimum Bactericidal Concentration (MBC) of red propolis ethanolic extract (RPE) was 4.66 mg/mL (0.4%) against S. mutans and 8.92 mg/mL (0.8%) (MIC) and 17.85 mg/mL (MBC) against L. casei
Red propolis extract showed antibacterial activity against the tested bacterial strains, exhibited acceptable cytotoxicity and reduced colonization of S. mutans and L. casei in a membrane disk biofilm model similar to chlorhexidine
Summary
Dental caries results from surface demineralization caused by an organized biofilm exposed to fermentable carbohydrates from the diet [1]. These carbohydrates act as nutrients for biofilm bacteria, especially acidogenic and aciduric species. Streptococcus mutans and Lactobacillus spp. are the main microorganisms responsible for the onset and progression of caries, respectively [2]. Both are able to proliferate and survive in acidic media, resulting in an elevated potential for caries development [3,4]. Propolis is a resinous substance originated from botanical compounds and collected by bees, and can be classified into different types (as green, brown and red) according to the chemical composition and geographical origin [12,16,17]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
