Abstract
Objective To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. Methods The methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. The antibacterial effect of the extracts against Enterococcus faecalis was evaluated using the agar diffusion technique. The minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxicity using the cellular line MDCK. Results The methanolic extract with the most antibacterial effect during the first 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, evidencing a growth inhibition zone of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. The MIC for both extracts was 75 µg/ml. The bactericidal effect of the Azadirachta indica extract was found at a concentration of 25 µg/ml and a concentration of 75 µg/ml for Moringa extract. Conclusions In conclusion, we demonstrated that the methanolic extract of Azadirachta indica and Moringa oleifera both have an antibacterial effect against Enterococcus faecalis strains during the first 24 and 48 hours. None of the extracts exhibited toxicity against the cell lines under low concentrations.
Highlights
One of the main objectives of endodontics is root canal disinfection, mainly associated with the colonization of anaerobic bacteria. ere are endodontic treatments that fail due to a complex root canal anatomy or antibiotic resistance of some bacterial families, with Enterococcus faecalis being the most resistant bacteria reported [1,2,3,4,5,6].Various antibacterial agents have been previously studied, and they can be obtained from natural sources or synthetic agents [7]
Azadirachta indica known as neem and Moringa oleifera known as moringa are both native Indian trees known for their high medicinal values due to their curative properties [9,10,11,12,13,14,15]
M. oleifera has been reported to have antiviral, International Journal of Dentistry antioxidant, antisclerotic, antibacterial, and antiinflammatory properties. It has been used as treatment for malaria, malnutrition, colon cancer, and myeloma [20]. Even though these plants have been studied to treat the previously mentioned ailments, no research evaluates if time is directly correlated with the antibacterial effect of A. indica against E. faecalis
Summary
To evaluate antibacterial and cytotoxic effect of 2 methanolic extracts of Azadirachta indica and Moringa oleifera against strains of Enterococcus faecalis (ATCC 29212) in vitro. E methanolic extracts of Azadirachta indica and Moringa oleifera were prepared in vitro. E antibacterial effect of the extracts against Enterococcus faecalis was evaluated using the agar diffusion technique. E minimum inhibitory concentration (MIC) was determined using the microdilution method and the cytotoxicity using the cellular line MDCK. E methanolic extract with the most antibacterial effect during the first 24 and 48 hours against Enterococcus faecalis was Moringa oleifera, evidencing a growth inhibition zone of 35.5 ± 1.05 and 44.83 ± 0.98, respectively. We demonstrated that the methanolic extract of Azadirachta indica and Moringa oleifera both have an antibacterial effect against Enterococcus faecalis strains during the first 24 and 48 hours. None of the extracts exhibited toxicity against the cell lines under low concentrations
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