Antibacterial and antioxidant activity of methanol extract of Evolvulus nummularius.

Rama S Verma,P S Pavithra,N Sreevidya

doi:10.4103/0253-7613.58514

Rama S Verma, P S Pavithra + Show 1 more

Open Access

https://doi.org/10.4103/0253-7613.58514

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Abstract

Objective:To evaluate the antibacterial and antioxidant activity of methanol extract of Evolvulus nummularius (L) L.Materials and Methods:Disc diffusion and broth serial dilution tests were used to determine the antibacterial activity of the methanol extract against two Gram-positive bacterial strains (Bacillus subtilus NCIM 2718, Staphylococcus aureus ATCC 25923) and three Gram-negative bacterial strains (Pseudomonas aeruginosa ATCC 27853, Klebsiella pneumoniae ATCC 70063 and Escherichia coli ATCC 25922). The methanol extract was subjected to preliminary phytochemical analysis. Free radical scavenging activity of the methanol extract at different concentrations was determined with 2, 2-diphenyl-1picrylhydrazyl (DPPH).Results:The susceptible organisms to the methanol extract were Escherichia coli (MIC=12.50 mg/ml) and Bacillus subtilus (MIC=3.125 mg/ml) and the most resistant strains were Staphylococcus aureus, Klebsiella pneumoniae and Pseudomonas aeruginosa. The methanol extracts exhibited radical scavenging activity with IC50 of 350 μg/ml.Conclusion:The results from the study show that methanol extract of E.nummularius has antibacterial activity. The antioxidant activity may be attributed to the presence of tannins, flavonoids and triterpenoids in the methanol extract. The antibacterial and antioxidant activity exhibited by the methanol extract can be corroborated to the usage of this plant in Indian folk medicine.

Highlights

In an attempt to screen plants growing in the forest of our campus for medicinal properties, we studied the antibacterial and antioxidant properties of methanol extract of E. nummularius
The phytochemical analysis of the methanol extracts performed in the present study showed the presence of alkaloids, triterpenoids, tannins, cardiac glycosides and flavonoids
Higher concentration of methanol extracts was needed for bactericidal action: The minimum bactericidal concentration (MBC) values for E. coli and B. subtilus were found to be 25 mg/ml and 50 mg/ ml, respectively

Summary

Introduction

In an attempt to screen plants growing in the forest of our campus for medicinal properties, we studied the antibacterial and antioxidant properties of methanol extract of E. nummularius. Microorganisms The antibacterial activity of methanol extract was determined by individually testing on Gram-positive and Gram-negative bacterial strains. Antibacterial activity assay The antibacterial activity of methanol extract was determined by disc diffusion and broth dilution method.[9] Nutrient Agar (NA) and Muller Hinton broth (MHB) were used for the tests.

Results

Conclusion