Abstract
Seaweeds have ecological functions as primary producers in marine waters. It also has an important economic value as a producer of hydrocolloids (alginate, agar and carrageenan) that is used in various industries of food and pharmaceuticals. This study aimed to determine the antibacterial and antioxidant activity of green algae Halimeda gracilis. The study was conducted in several stages, sample collection and preparation, extraction of bioactive compound, fractionation, antibacterial and antioxidant test, and phytochemical. Extraction was done by maceration method using methanol and concentrated by rotary evaporator. The methanol extracts of H. gracilis were tested against Staphylococcus aureus and Escherichia <br />coli. Methanol extract of H. gracilis formed inhibition zone against the test bacteria with diameter of inhibition zone was 10 mm and 6 mm, respectively. After liquid-liquid partition (water: ethyl acetate), inhibition zone was only seen in the ethyl acetate fraction of H. gracilis with diameter of inhibition zone was 6 mm and 7.50±1.71 mm, respectively. Antioxidant test methanol extracts and ethyl acetate fractions of H. gracilis each show IC50 value of 290.49 ppm and 375.50 ppm. Phytochemical test showed methanol extract of H. gracilis contains phenols and steroids.<br /><br />
Highlights
Rumput laut merupakan salah satu produsen primer di ekosistem perairan laut bersama dengan fitoplankton, lamun, dan mangrove
Seaweeds have ecological functions as primary producers in marine waters. It has an important economic value as a producer of hydrocolloids that is used in various industries of food and pharmaceuticals
The study was conducted in several stages, sample collection and preparation, extraction of bioactive compound, fractionation, antibacterial and antioxidant test, and phytochemical
Summary
Bahan baku yang digunakan pada penelitian ini adalah rumput laut hijau H. gracilis yang diperoleh dari perairan Pulau Karya, Kepulauan Seribu, Jakarta Utara. Bahan lainnya yaitu Media Nutrient Broth (NB) (Oxoid), Mueller Hinton Agar (MHA) (Oxoid), Nutrient Agar (NA) (Oxoid), radikal 1,1-diphenyl-2-picryl-hydrazyl, metanol, etil asetat, n-heksana, kloroform, akuades, dan antibiotik kloramfenikol. Peralatan yang digunakan dalam penelitian ini diantaranya freeze dryer FDU1200 Eyela, pipet mikro (Eppendorf), shaker (Heidolph VV2000), timbangan digital (Sartorius TE214S), vortex (Pasolina type NS8), rotary evaporator Buchi R-114, inkubator Yamato IS900, autoklaf Yamato SM52, dan spektrofotometer Shimadzu UV-1800
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