ANTIBACTERIAL AND ANTIOXIDANT ACTIVITIES OF STEM BARK ESSENTIAL OIL CONSTITUENTS OF LITSEA GLUTINOSA C. B. ROB.

Arunodaya H. S.,Shashikumar R.,Girish Kumar K.,Krishna V.

doi:10.22159/ijpps.2016v8i12.13577

Arunodaya H. S., Shashikumar R. + Show 2 more

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https://doi.org/10.22159/ijpps.2016v8i12.13577

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Abstract

Objective: To evaluate the chemical composition, antibacterial and antioxidant properties of stem bark essential oil of Litsea glutinosa C. B. Rob.Methods: The essential oil isolated from stem bark of L. glutinosa and their chemical composition was analyzed by gas chromatography coupled with mass spectrometry detector. The in vitro antibacterial activity of the stem bark essential oil was investigated against eight human pathogenic bacterial clinical isolates using agar disc diffusion method and MIC value was determined by modified resazurin microtitre-plate assay. The antioxidant activity of essential oil was measured by 2, 2-diphenyl-1-picrylhydrazyl radical (DPPH), 2, 2-azinobis-3-ethylbenzothiazoline-6-sulphonate radical cation (ABTS) and β-carotene bleaching assay.Results: GC-MS analysis of stem bark essential oil resulted in the identification of 37 compounds, off which 9,12-octadecadienoic acid (62.57%), hexadecanoic acid (12.68%), stigmast-5-en-3-ol (6.87%) and vitamin E (2.51%) were the main constituents representing 84.63% of the oil. The determination of in vitro antibacterial activity of stem bark essential oil resulted in significant inhibition zone (15.00±0.57 mm) and MIC value (0.15±0.15×10-2 mg/ml) against the pathogenic bacteria Vibrio cholera followed by Pseudomonas aeruginosa and Salmonella typhi. The results of DPPH radical scavenging (IC50:4.540±0.06 µg/ml), ABTS (IC50:256.02±0.06 µg/ml) and β-carotene bleaching assay (%I: 78.51±0.42 %) showed significant in vitro antioxidant property.Conclusion: L. glutinosa stem bark essential oil showed potential antibacterial activity against the Vibrio cholera. The results of this investigation supported the ethnomedical claim of essential oil as a demulcent, antidiarrheal and antioxidant drug.

Highlights

Litsea glutinosa is an endemic and threatened aromatic medicinal tree which belongs to the Lauraceae family and found to be very sparsely distributed in the Western Ghats of Karnataka State, India
MO, USA), butylated hydroxyl anisole (BHA), butylated hydroxytoluene (BHT), trichloroacetic acid (TCA), folin–ciocalteu reagent, dimethyl sulfoxide (DMSO) were purchased from Merck limited (Mumbai, India), resazurin, hi sensitivity broth, β-carotene, potassium persulfate, linoleic acid, ascorbic acid, nutrient agar (NA) media, sodium sulfate, gentamicin and ampicillin dish were procured from Himedia Pvt
The amount of essential oil obtained from the stem bark of L. glutinosa was 2.3 ml/100 g dry weight, with characteristic aroma of allspice (Pimenta dioica)

Summary

Introduction

Litsea glutinosa is an endemic and threatened aromatic medicinal tree which belongs to the Lauraceae family and found to be very sparsely distributed in the Western Ghats of Karnataka State, India. Leaves and stem bark essential oil possess allspice aroma. Traditional medicinal practitioners residing in the vicinity of Bhadra Wild Life Sanctuary are using L. glutinosa stem bark oil as the demulcent and mild astringent for diarrhea and dysentery. It is reported for relieving pain, arousing sexual power, aches, sore eyes, skin infections, gouty joints, wounds and for producing a soothing effect on the body [1,2,3]. The berries oil is used in the treatment of rheumatism and shampoo preparation [5]

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