Abstract
Human pathogens are rapidly acquiring resistance to antibiotics leading to treatment failure. We carried out this study to isolate and screen actinomycetes strains that have potential to kill bacterial and fungal pathogens. In this descriptive study 288 soil and water samples were processed by standard microbiological techniques at Central Department of Microbiology,Tribhuvan University from 2013 to 2015. Screened actinomycetes were cultivated for bioactive metabolite production and minimum inhibitory concentration (MIC) of metabolites were determined against bacterial pathogens including multidrug resistant bacteria and fungi. One hundred twenty isolates having antimicrobial property were screened. Out of them, four most potent strains, Nocardiopsis prasina, Streptomyces violarus, Streptomyces krainskii and Streptomyces tsusimaensis were identified all having both antibacterial and anti-fungal property.Highest zone of inhibition (ZOI)was given by N. prasina against Candida albicans(41.33 ±1.15mm) and among bacteria, maximum ZOI was against Acinetobacter baumannii(31.33±3.05mm). MIC value of metabolite of N.prasina was 0.125mg/ml for E.coli and C. albicans. It was 2.5 mg/ml each for methicillin resistant Staphylococcus aureus (MRSA), A. baumannii and Salmonella Typhi and 0.625 mg/ml for Bacillus Subtilis. Bioactive metabolite producing actinomycetes were recovered from soil and tested against human pathogenic bacteria and fungiand found to have antibacterial and antifungal property.
Highlights
Antimicrobial resistance creates a great threat for effective prevention and control of several diseases caused by bacteria, fungi, viruses and other parasites
The strains showing antimicrobial property were cultivated under optimum conditions in starch casein broth at 30oC for 7 days at 150 rpm and bioactive metabolites were extracted in ethylacetate
The extracts were subjected to secondary screening against Bacillus subtilis, methicillin resistant Staphylococcus aureus (MRSA), E.coliATCC25922, Acinetobacter baumannii(MDR), Salmonella Typhi(MDR) and Candida albicans by agar well diffusion method.[10]
Summary
Antimicrobial resistance creates a great threat for effective prevention and control of several diseases caused by bacteria, fungi, viruses and other parasites. Vancomycin resistant enterococci, MRSA, fluoroquinolone resistant Salmonella, Campylobacter and Shigella spp. along with many other multidrug resistant pathogens create problem so that new antibiotics should be developed to address resistance problem.[2] Antibiotics are bioactive secondary metabolites produced by bacteria, fungi and plants. Human pathogens are rapidly acquiring resistance to antibiotics leading to treatment failure.We carried out this study to isolate and screen actinomycetes strains that have potential to kill bacterial and fungal pathogens
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
