Antibacterial and Antifungal Potential of Berberine Isolated from Stem of Berberis aristata DC

Abstract

Background: This research was conducted to evaluate berberine as an isolated bioactive compound from Berberis aristata DC for its activity against microbial infections. To examine the antibacterial activity using ethanol hot extraction procedure to verify the medicinal use of B. aristata DC in infection control and qualitatively estimate the phytochemical composition of the plant stem extract. Objective: To isolate a bioactive phytoconstituent from B. aristata DC for its antimicrobial efficacy in managing human infections. Methods: The antibacterial activity of berberine derived from the stem extract was examined against gram-ve and +ve multi-drug resistance examined by diffusion method using an agar-well isolated from the stem extract was examine by the agar-well using a 96-well microtitre plate. The minimum inhibitory concentration was calculated against each bacterium using both the microtitre plate technique and broth dilution of berberine Results: The appearance of alkaloids in the methanol extract of the stems was demonstrated. The pathogenic bacteria used were Shigella sp., Escherichia coli, including some more standard bacterial strains. These multi-drug resistant (MDR) bacteria were reported with bacterial inhibition capability when methanol was used as a solvent. In addition, the effect of berberine against Shigella sp. was the maximum inhibition zone size (1.25 cm). Ciprofloxacin 30 μg/disc served as the reference/ +ve control, whereas 10% dimethyl sulfoxide (DMSO) was the -ve control. These chosen bacteria were reported minimum inhibitory concentration (MIC) of berberine values ranging from 200 to 1000 μg/mL. Conclusions: B. aristata DC stem extract berberine showed antibacterial properties using hot methanol against all bacteria. In alternative and complementary medicine, berberine is used to treat diseases caused by many strain of bacteria.