Abstract
Pediococcus pentosaceus LBM 18 has shown potential as producer of an antibacterial and antifungal bacteriocin-like inhibitory substance (BLIS). BLIS inhibited the growth of spoilage bacteria belonging to Lactobacillus, Enterococcus and Listeria genera with higher activity than Nisaplin used as control. It gave rise to inhibition halos with diameters from 9.70 to 20.00 mm, with Lactobacillus sakei being the most sensitive strain (13.50–20.00 mm). It also effectively suppressed the growth of fungi isolated from corn grain silage for up to 25 days and impaired morphology of colonies by likely affecting fungal membranes. These results point out that P. pentosaceus BLIS may be used as a new promising alternative to conventional antibacterial and antifungal substances, with potential applications in agriculture and food industry as a natural bio-controlling agent. Moreover, cytotoxicity and cell death induction tests demonstrated cytotoxicity and toxicity of BLIS to human colon adenocarcinoma Caco-2cells but not to peripheral blood mononuclear cells, with suggests possible applications of BLIS also in medical-pharmaceutical applications.
Highlights
Pediococcus pentosaceus LBM 18 has shown potential as producer of an antibacterial and antifungal bacteriocin-like inhibitory substance (BLIS)
L. sakei and E. faecium were overnight cultivated at 37 °C in MRS medium, while Listeria strains in Brain Heart Infusion (BHI) medium under the same conditions
The ability of Pediococcus pentosaceus LBM 18 to produce bacteriocin-like inhibitory substance (BLIS) and its effectiveness as an antimicrobial agent were first evaluated by the agar well diffusion assay
Summary
Pediococcus pentosaceus LBM 18 has shown potential as producer of an antibacterial and antifungal bacteriocin-like inhibitory substance (BLIS). It gave rise to inhibition halos with diameters from 9.70 to 20.00 mm, with Lactobacillus sakei being the most sensitive strain (13.50–20.00 mm) It effectively suppressed the growth of fungi isolated from corn grain silage for up to 25 days and impaired morphology of colonies by likely affecting fungal membranes. They are Gram-positive, non-pathogenic, non-sporulating, facultative anaerobic, catalase negative and acid tolerant bacteria with a strictly fermentative m etabolism[3,6], which are often used as industrial starter cultures in food fermentation technology[7,8] Their metabolic products are recognized as Generally Regarded as Safe (GRAS) by the Food and Drug Administration (FDA) and recommended by the Qualified Presumption of Safety (QPS) list of the European Food Safety Authority (EFSA)[9]. Nisaplin has a broad spectrum of action against Gram-positive bacteria, including LAB34, but, generally, it is hardly effective against Gram-negative bacteria, molds and yeasts[30,31]
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
