Abstract
A total of (213) canned food samples comprising of Tuna &Sardines,Juices, Tomatoes pasts, Jam and Beef were randomly collected fromsuper stores and local markets in Tanta city from Awlad Ragab,Fatthallah, Munshwi, Casion. Also 24 fast food samples collected fromlocal cafeterias and restaurants in Tanta city from Al Gaan, Abu Deshish,Al Baraka, Abu Owaf. All canned food samples were within expiry date,none of which is bloated, leaking and/or physically damaged. Sampleswere investigated for some bacteria and fungi using specific media andincubated for suitable incubation period. The results revealed thatspecies of microbes isolated in this study namely Bacillus subtilis,Bacillus cereus, Bacillus atrophaeus., Staphylococcus saprophyticus,Enterococcus faecalis, Staphylococcus epidermides, E. coli, Klebsiella,Salmonella , Aspergillus niger, Penicillium notatum, Candida tropicalisand Saccharomyces cerevisiae. Biochemical tests were performed for allisolates to know the most common isolates (Bacillus cereus andAspergillus niger). Antimicrobial activities of chitosan were investigatedagainst the most common isolates B. cereus and A. niger. Resultsrevealed that chitosan has antibacterial activity towards Bacillus cereus.The minimum inhibition concentration (MIC) for chitosan was 6.25μg/ml with mean diameter of inhibition zone 8 mm. Also chitosan hasantifungal activity toward Aspergillus.niger with minimum inhibitionconcentration (MIC) 30mg/ml and percent of fungal growth inhibition16.7%.
Highlights
Canned foods are foods that packed in hermetically sealed containers and become sterile through packing
Fast food are foods which prepared in cafeteria or related food restaurants and immediately consumed, this fast food may contain food eaten raw like salads, spices which are favorable media for microbial contamination by pathogenic and spoilage microbes especially in crowded restaurants and from suppliers, so to improve safety of these food products, the associated stuff need to be sure for good manufacturing practices from food suppliers and food workers [3]
The information on the container/labels was recorded to include manufacture and expiry dates, manufacturer’s address, 24 fast food samples were collected from local cafeteria and restaurant in Tanta city
Summary
Canned foods are foods that packed in hermetically sealed containers and become sterile through packing. Canning leads to kill harmful microbes in food, if canning processing were performed improperly, canned food become available media for microbial contamination by different microbes which may be harmful for consumers if they increase in number or leads to toxicity. This contamination may occur during preprocessing, processing or after processing, may be due to physical causes like defective containers, improperly closed cans or bad packing or transportation [1]. Contamination in food industry by storage fungi like Aspergillus and Pencillium is of great concern because of secondary metabolites produced by these fungi such as mycotoxins that has a bad effect on human health [6]. Many researches show that the antibacterial activity of chitosan effective than antifungal activity [18, 19]
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