Antibacterial and anticancerous biocompatible silver nanoparticles synthesised from the cold-tolerant strain of Spirulina platensis

Abstract

The new age drugs are nanoparticles of polymers, metals or ceramics are now extensively utilized in modern medicine and healthcare which can combat conditions like cancer and fight human pathogens. Nanomaterials based on biological origin is highly appreciable because of less toxicity, high efficacy and biocompatibility. In the present study, silver nanoparticles were synthesized from the biomass of cold tolerant strain of Spirulina platensis and the synthesized nanoparticles were evaluated against antibacterial and anti cancer activity. Silver nanoparticles were synthesized by the algal culture and characterized by UV-visible spectroscopy, Fourier transform infrared spectroscopy (FTIR), field emission scanning electron microscopy (FE-SEM) and X ray diffraction (XRD) studies. Anti bacterial activity has been studied with free nanoparticles adopting agar diffusion assay, biofilm inhibition assay and nanoparticles fabricated wound dressing against representative gram negative organism Pseudomonas aeruginosa and gram positive organism Staphylococcus aureus respectively. The in vitro anti cancer activity of silver nanoparticles were screened against human Hep 2 cell lines by means of MTT assay. Reduction of silver ions by the algal culture was observed during 72 hours of incubation and the synthesized nanoparticles were further characterized. Anti bacterial study reveals both the strains were susceptible to free nanoparticles and fabricated wound dressing treatment. The in vitro anti cancer activity of silver nanoparticles were screened against human Hep 2 cell lines by means of MTT assay which reveals that cell viability has been reduced as dose dependent manner. The observed results imply that silver nanoparticles synthesized from Spirulina platens is cold tolerant strain can be used as potential anti bacterial and anti cancerous agent.