Abstract
Herein, Ag nanoparticles were prepared by fish bile juice as a reducing and capping agent. Then Ag/ graphene oxide (GO) nanocomposite was fabricated. The nanocomposite (BJ-Ag0.6 @GO0.4) showed remarkable antibacterial activities against Gram-positive and Gram-negative pathogenic bacteria. The MIC values of the nanocomposite were recorded as 10 and 8 µg/mL for S.aureus (MTCC 1430) and P. auregenosa (MTCC-1688), respectively. Antibacterial mechanism study of the synthesized nanocomposite excavates that, BJ-Ag0.6 @GO0.4 caused extensive membrane damage and cytoplasmic leakage, leading to cell’s metabolic arrest. Increased ROS generation is observed, causing disruption in the antioxidant defense mechanism of the bacterial cell. The synthesized nanocomposite also showed excellent destruction of preformed biofilm up to 96.52% and 61.23% for S.aureus (MTCC 1430) and P. auregenosa (MTCC-1688), respectively, at MIC concentration of the nanocomposite.
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