Year-end Sale % OFF 
Abstract
Background:Periodontitis, a chronic inflammatory disease, is the leading cause of tooth loss in adults. Evidence for the anti inflammatory activity of M. alba Stem Extract (MSE) in periodontal disease is limited.Objective:The study aimed to investigate the inhibitory effect of MSE on the growth of periodontopathic bacteria and expression of interleukin (IL)-6 and IL-8 in Porphyromonas gingivalis Lipopolysaccharide (LPS)-stimulated human Periodontal Ligament (hPDL) fibroblasts.Methods:The antimicrobial activities of MSE were tested against P. gingivalis and Aggregatibacter actinomycetemcomitans by the disk diffusion, the minimum inhibitory concentration and the minimal bactericidal concentration methods. Cytotoxicity of P. gingivalis LPS and MSE on hPDL fibroblasts was determined by MTS assay. The expression of cytokines (IL-6 and IL-8) mRNA and proteins in hPDL fibroblasts was measured using the reverse transcription-qPCR and enzyme-linked immunosorbent assay, respectively.Results: MSE exhibited antibacterial activities against P. gingivalis and A. actinomycetemcomitans with the zones of inhibition of 10.00 ± 0.33 mm and 17.33 ± 0.58 mm, respectively. MIC and MBC values for MSE against P. gingivalis were 62.5 μg/ml. The MIC and MBC values against A. actinomycetemcomitans were 250 μg/mL and 500 μg/ml, respectively. P. gingivalis LPS was shown to mediate the expression of pro-inflammatory cytokines in hPDL fibroblasts. However, treatment with MSE concentrations of 2.5 and 5.0 μg/ml significantly suppressed P. gingivalis LPS-induced IL-6 and IL-8 mRNA and protein expression (p< 0.05).Conclusion: The present study demonstrates that MSE has antibacterial activity against two putative periodontal pathogens. MSE suppressed IL-6 and IL-8 expression in P. gingivalis LPS-stimulated hPDL fibroblasts, indicating a possible anti-inflammatory effect. Thus, it is a potential adjunctive agent for the treatment of periodontitis.
Highlights
Periodontitis is characterized by inflammatory destruction of the supporting tissues of the teeth [1]
P. gingivalis LPS has been shown to stimulate the production of IL-6 and IL-8 by Human Periodontal Ligament (hPDL) fibroblasts [12, 13], suggesting that these mediators are involved in the pathogenesis of periodontitis
M. alba Stem Extract (MSE) showed potentially bactericidal activity against P. gingivalis with an Minimum Bactericidal Concentration (MBC) of 62.5 μg/ml while an MBC of A. actinomycetemcomitans reached to 500 μg/ml
Summary
Periodontitis is characterized by inflammatory destruction of the supporting tissues of the teeth [1]. P. gingivalis LPS acts as a potent stimulus to a variety of host cells, which subsequently results in the expression of inflammatory cytokines leading to the development and progression of the related host immune response in periodontal diseases [6]. Human Periodontal Ligament (hPDL) fibroblasts, the primary cultured cell of the ligament, are responsible for the formation, repair and maintenance of the extracellular matrix of the hPDL. These cells are involved in inflammatory and immune processes leading to cytokine (i.e., interleukin (IL)-1 or IL-6) or chemokine (i.e., IL-8) release which could further enhance inflammation [7, 8] and leading to periodontal destruction via Matrix Metalloproteinases (MMPs) [9]. Evidence for the anti inflammatory activity of M. alba Stem Extract (MSE) in periodontal disease is limited
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
