Antibacterial and anti-breast cancer cell line activities of Sanghuangporus sp.1 extracts

Kusavadee Sangdee,Benjaporn Buranrat,Aphidech Sangdee,Piyanoot Jaihan,Khwanyuruan Naksuwankul

doi:10.4314/tjpr.v16i3.16

Abstract

Purpose : To evaluate the activity of extracts of Sanghuangporus sp.1 fungus against pathogenic bacteria and a breast cancer cell line. Methods : The wild fruiting body and mycelium of Sanghuangporus sp.1 were extracted with water and ethanol by ultrasonication extraction. The activity of the extracts against pathogenic bacteria was determined by agar-well diffusion method while minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) were obtained by agar-well diffusion and broth macrodilution methods. The cytotoxicity of the extract against human breast cancer cell line MCF-7 was determined by sulforhodamine B assay. Results : Only the ethanol mycelial extract exhibited antibacterial activity. Activity was detected against 6 of the 17 test strains of bacterial pathogen. The MICs and MBCs against these 6 strains were quite low, especially for B. cereus ATCC 11778 (2.5 and 2.5 mg/mL) and S. aureus (MSSA, DMST 2933, 2.5 and 5.0 mg/mL). The ethanol mycelial extract was a more potently cytotoxic against MCF-7 cells than either the aqueous mycelial extract or the ethanol wild fruiting body extract, inhibiting cell growth at a concentration of 250 μg/mL. The aqueous wild fruiting body extract was inactive against MCF-7 cells when compared with untreated control groups. Conclusion : The ethanol mycelial extract of the medicinal mushroom Sanghuangporus sp.1, obtained by ultrasonication extraction method, exhibited potent antibacterial and anticancer activity and seems to be a substitute for wild Sanghuangporus sp. Keywords : Antibacterial activity, Anticancer activity, Sanghuangporus sp.1, Mycelial

Highlights

Mushrooms have been used as traditional remedies for many years [1], playing an important role in traditional medicine in Japan, China, Korea and other Asian countries [2]
The results showed that only the ethanolic mycelial extract exhibited antibacterial activity
The results showed that MCF-7 cells were more sensitive to the mycelial extracts than the wild fruiting body extracts

Summary

Introduction

Mushrooms have been used as traditional remedies for many years [1], playing an important role in traditional medicine in Japan, China, Korea and other Asian countries [2]. Hur et al [4] found that methanol extract from P. linteus inhibits the growth of methicillin-resistant Staphylococcus aureus (MRSA). Another medicinal mushroom in this genus is P. igniarius. The extracts from P. gilvus, P. rimosus and P. badius could inhibit growth of the fungus Alternaria alternata [6]. Extracts from P. baumii have activity against influenza and methicillin-resistant S. aureus [15,16]. These findings indicate that many fungi in the genus Phellinus produce bioactive compounds, and represent a potential source of new therapeutic agents. Some of the above species have recently been reclassified, including P. linteus, which has been moved from the Phellinus genus to the Sanghuangporus genus, and is known as Sanghuangporus linteus

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