Abstract
Amphiphilic copolymers are recognized as important biomaterials and used as antibacterial agents due to their effective inhibition of bacterial growth. In current study, the amphiphilic copolymers of P(DMAEMA-co-MMA) were synthesized using free radical polymerization by varying the concentrations of hydrophilic monomer 2-dimethylamino ethylmethacrylate (DMAEMA) and hydrophobic monomer methyl methacrylate (MMA) having PDI value of 1.65–1.93. The DMAEMA monomer, through ternary amine with antibacterial property optimized copolymers, P(DMAEMA-co-MMA), compositions to control biofilm adhesion. Antibacterial activity of synthesized copolymers was elucidated against Gram-positive Staphylococcus aureus (ATCC 6538) and Gram-negative Escherchia coli (ATCC 8739) by disk diffusion method, and zones of inhibition were measured. The desired composition that was PDM1 copolymer had shown good zones of inhibition i.e., 19 ± 0.33 mm and 20 ± 0.33 mm for E. coli and S. aureus respectively. The PDM1 and PDM2 have exhibited significant control over bacterial biofilm adhesion as tested by six well plate method. SEM study of bacterial biofilm formation has illustrated that these copolymers act in a similar fashion like cationic biocide. These compositions viz. PDM1 and PDM2, may be useful in development of bioreactors, sensors, surgical equipment and drug delivery devices.
Highlights
Accepted: 20 December 2020Biofouling is the growth of microbes on exterior of material that initiated by protein adsorption or microorganisms on the surface of substrate that is a ubiquitous challenge for a number of bio medical applications [1,2]
In this study we report a facile approach to enhance the antimicrobial activity of amphiphilic copolymers by varying concentration of hydrophilic and hydrophobic monomers
Traces of symmetric GPC distribution showed the uniformity of the copolymers via free radical polymerization
Summary
Biofouling is the growth of microbes on exterior of material that initiated by protein adsorption or microorganisms on the surface of substrate that is a ubiquitous challenge for a number of bio medical applications [1,2]. The unregulated attachment of microbes on implant materials surface is an undesirable phenomenon that causes infection and degradation of the function of medical instruments [5,6]. Most commonly used antimicrobial reagents for bio medical applications can be classified into four specific categories that include organic agents such as formaldehyde and isothiazolones, oxidants that include chlorine and peroxides, electrophilic agents such as mercury, copper and silver, cationic active compounds such as quaternary ammonium and chlorhexidine [10,11]. Different antifouling functional groups such as quaternary ammonium group, Published: 9 January 2021
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