Antibacterial Activity of TiO2 Nanoparticles Prepared by One-Step Laser Ablation in Liquid

Khawla S Khashan,Farah A Abdulameer,Salim Albukhaty,Mohammed A Ibrahem,Tahani Al-Muhimeed,Abeer A Alobaid,Ghassan M Sulaiman

doi:10.3390/app11104623

Khawla S Khashan, Farah A Abdulameer + Show 5 more

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https://doi.org/10.3390/app11104623

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Abstract

Laser ablation in liquid was utilized to prepare a TiO2 NP suspension in in deionized distilled water using Q-switch Nd: YAG laser at various laser energies and ablation times. The samples were characterized using UV–visible absorption spectra obtained with a UV–visible spectrophotometer (UV-Vis,) Fourier transform infrared (FTIR), X-ray diffraction (XRD), and transmission electron microscope (TEM). While, UV-Vis spectra showed the characteristic band-to-band absorption peak of TiO2 NPs in the UV range. FTIR analysis showed the existence of O-Ti-O bond. XRD patterns indicated the presence of (101) and (112) plane crystalline phases of TiO2. TEM images showed a spherical-like structure of TiO2 NPs with various size distributions depending on the ablation period. It was also found that there is a relationship between laser ablation time and TiO2 NP size distribution, where longer ablation times led to the smaller size distribution. The antibacterial activity of TiO2 NPs was evaluated with different species of bacteria such as Escherichia coli, Pseudomonas aeruginosa, Proteus vulgaris, and Staphylococcus aureus, using the liquid approach. The optimum activity of TiO2 NPs is found to be against E. coli at 1000 μg mL−1. Furthermore, adding, TiO2 NPs (1000 μg mL−1) in the presence of amoxicillin has a synergic effect on E. coli and S. aureus growth, as measured by the well diffusion method. However, both E. coli (11.6 ± 0.57mm) and S. aureus (13.3 ± 0.57mm) were inhibited by this process.

Highlights

The risk of biological and bacterial attacks has grown enormously in the last decade or so, especially in the human consumption sectors such as food, food packaging and water.This increasing risk stimulates scientists to develop new inorganic antibacterial nanoparticle substances that have no side effects and are easy to implement
The results showed a gradual increase in the NP mass concentration with laser energy, most likely due to target evaporation at higher temperatures caused by high energy
Laser ablation in liquid is a powerful and efficient technique to synthesize TiO2 NPs with a spherical shape and various sizes suspended in deionized distilled water

Summary

Introduction

The risk of biological and bacterial attacks has grown enormously in the last decade or so, especially in the human consumption sectors such as food, food packaging and water This increasing risk stimulates scientists to develop new inorganic antibacterial nanoparticle substances that have no side effects and are easy to implement. Reducing the dimensions of certain materials, such as metal oxide semiconductors, to the nanoscale size could result in a wholly new material that has optical and/or electronic properties different from its bulk material This enables scientists to explore the benefits of nanomaterials in a wide range of scientific fields such as biomedicine, optoelectronics, and the environment [1,2,3,4,5,6]. It has a unique photocatalytic activity and good thermal stability and biocompatibility to chemicals

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