Abstract
Active compound extracted from Casia alata leaf was obtained from methanol extract and ethylacetat fraction. Based on research, anti bacteria activity of methanol extract of Casia alata at concentration 6 mg/mL gave high inhibition of the growth of positive gram bacteria colonies of B. cereus and S. aureus with inhibition zone 11,0 mm and 16,1 mm and gaves negative inhibiton of negative gram bacteria colonies of E. coli and S. typhi with inhibition zone 11,6 mm dan 5,9 mm. Ethylasetat fraction showed higher zone inhibition then methanol extract. Based on research result it was show that ethylacetat fraction gaves strong inhibition of the growth of positive gram bacteria colonies of B. cereus and S. aureus with inhibition zone 18,1 mm and 14,3 mm, and gave moderate inhabitation for negative gram bacteria colonies of coli and S. typhi with inhibition zone 9,0 mm and 5,7 mm. Separation result of ethylacetat fraction using coloumn chromatography with silica gel G60 F254 Âas stationary pahase showed that ethylacetat fraction with the same concentration at 6 mg/mL show higher inhibition effect especially for positive gram bacteria colonies of B. cereus and S. aureus with inhibition zone 24,2 mm and 24.0 mm.
 Key Word: Casia alata, ethylacetat ftaction, antibacteria activity
Highlights
Active compound extracted from Casia alata leaf was obtained from methanol extract and ethylacetat fraction
Part of plant that being used was the leaf of Cassia alata
Chrpmatgraphy electronic balance, ultra violet light, ultra sonic and other tools that commonly used in research laboratorium of organic chemistry
Summary
Before it put into macerator, simplisia was crude using grinder to enlarge it surface area. Simplicia that has been refined was put into macerator while adding the methanol until all part of simplicia are fully submerged. Filtrate that was filtered were mix and evaporate it using rotary evaporator with temperature is 400 C until it became viscious extract and after that, extract was weighed. Methanol extract of Casia alata being partitioned with hexan and distilled water (1:1), water layer that form was taken being partition using ethylacetat. This ethylacetat fraction of Casia alata leaf than evaporate back using rotary evaporator so crude ethylacetat were obtained
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
