Antibacterial Activity of Synergistic Effect of colicin and Gold Nanoparticles against Klebsiella pneumonia

Abstract

Fifty of urine samples were collected from patients with urinary tract infection (UTI). The samples were collected from AL-Yarmuk hospital in Baghdad. All of the isolates were diagnosed using biochemical test and vitek. The result showed that 30 (60%) isolates identified as E.coli from 50 urine samples. The colicinogenic isolates were determined using cup assay methods. The results showed that 10 out of 30 isolates (33.3%) were detect as colicin producers from 30 isolate identified as E.coli depending on the clear zone that observed against the sensitive isolate. Colicin was extracted from the efficient isolate. Colicin activity (320 U/ml) was determined by well assay method. The protein concentration (520 μg/ml) estimated by using Bradford assay. The watery extract of Chilli papers (Capsicum baccatum) was extracted and used it as reducing and capping agent for gold nanoparticles synthesis. The characterization of the gold nanoparticles was done by UV-Visible Spectrophotometer, Transmission Electron Microscope (TEM), and resulting spherical nanoparticles with diameter ranging between (35–70 nm). The antibacterial activity of colicin alone and gold nanoparticles alone and combination of colicin with gold nanoparticles against ten isolates Klebsiella pneumonia isolated from urine samples, using tube method.