Abstract
In this study, antibacterial activity of ethanol extract and essential oil of 10 Iranian folklore herbs including, Heracleum lasiopetalum Boiss., Satureja bachtiarica Bunge., Thymus daenensis Celak., Ziziphora teniur L., Echiophora platyloba L., Dracocephalum multicaule Benth., Kelussia odoratissima Mozff., Mentha longifolia Hudson., Achillea kellalensis Boiss. and Stachys lavandulifolia Vahl. were investigated against of Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae by agar disc diffusion and serial dilution assays. Most of the extracts and essential oils showed relatively high antibacterial activity against all the tested bacteria with the diameter of inhibition zone ranging between 8 and 23 mm. Of the plants studied, the most active extracts were those obtained from essential oil of Satureja bachtiarica and Thymus daenensis. The MIC values for active extract and essential oil ranging between 0.039 and 10 mg/ml. The results obtained appeared to confirm the antibacterial potential of the plants investigated. In conclusion it can be said that the extracts and essential oils of Satureja bachtiarica and Thymus daenensis could be used as natural antibacterial agents in the food preservation and human health.
Highlights
Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella, Streptococci and etc. are common food-borne pathogenic bacteria and are frequently isolated from various foods, including meat, milk and milk products, seafood and vegetables
The Growth inhibition value of extract and essential oil on bacteria strains are shown in table 2
The results showed that Gram- negative bacteria (Escherichia coli, Pseudomonas aeruginosa and Klebsiella pneumoniae) were more sensitive than Gram-positive bacteria (Staphylococcus aureus) (Table 2-4)
Summary
Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa, Klebsiella pneumoniae, Salmonella (over 1600 types), Streptococci and etc. are common food-borne pathogenic bacteria and are frequently isolated from various foods, including meat, milk and milk products, seafood and vegetables. Are common food-borne pathogenic bacteria and are frequently isolated from various foods, including meat, milk and milk products, seafood and vegetables. Food poisoning originating from contaminated foods by both Gram-positive and Gram-negative bacteria causes concern to society and to the industry. Medicinal herbs contain physiologically active principles that over the www.ccsenet.org/ijb years have been exploited in traditional medicine for the treatment of various ailments as they contain anti-microbial properties (Kelmanson et al, 2000; Srinivasan et al, 2001). Antimicrobial properties of herbs have been documented in ancient literature and the interest continues to the present. Few of these have been investigated for their antimicrobial
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