Abstract
The effect of a crude, organic, non-alkaloid extract derived from R. stricta leaves were evaluated against methicillin-resistant Staphylococcus aureus (MRSA) pathogens. Antibacterial activities were determined by zones of inhibition using a new 1% agarose well-diffusion method. Structural and morphological alterations of MRSA cells were investigated using transmission electron microscopy. The extract showed antibacterial activities against MRSA pathogens. The extract inhibited growth, with zones of inhibition ranging between 6 and 19 mm. Transmission electron microscopy revealed that the extract distorts the cellular architecture of MRSA pathogens. Crude organic non-alkaloid extract derived from R. stricta leaves might serve as a novel antibiotic agent to prevent or treat lifethreatening MRSA infections.
Highlights
One of the most problematic and economically relevant nosocomial pathogens in the world is Methicillin-resistant Staphylococcus aureus (MRSA) [1]
In earlier studies from our laboratory, a significant amount of work has been done on examining the alkaloid extracts of R stricta, the aim of this study was to explore the antibacterial activities of organic, non-alkaloid extract derived from R. stricta leaves against methicillin-resistant Staphylococcus aureus (MRSA) using a 1% agarose well-diffusion method as well as transmission electron microscopy (TEM)
One Determination of antibacterial activity using Agarose well diffusion method The antibacterial activity of the extract depended on the concentration of the test material as well as on the quality of the solid base media
Summary
One of the most problematic and economically relevant nosocomial pathogens in the world is Methicillin-resistant Staphylococcus aureus (MRSA) [1]. The problem has been intensified in last two decades as multidrug-resistant MRSA has emerged with resistance to a wide range of antibiotics and antimicrobial agents [2]. Since 1961, MRSA continues to be a life-threatening MDR bacterium, changing its resistance patterns by acquiring resistance to each new antibiotic used against it. MRSA is a causative agent of many infectious diseases of the skin, soft tissue, respiratory tract, bones and joints, surgical wounds, urinary tract, and bloodstream. Such infections are challenging to treat because they are resistant to several commonly used antibiotics [3]
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