Antibacterial Activity of Probiotics Cell-Free Fermentation Filtrate from Passiflora edulis Sims. againts Pathogen bacteria

Abstract

Fermentation of probiotics (MM1, MM2, and MM3) isolated from red passion fruit (Passiflora edulis Sims.) in De Man Rogose Sharpe broth medium has been performed. Determination of antibacterial activity of probiotics cell free fermentation filtrate (PCFFF) against Mycobacterium tuberculosis H37Rv has been carried out. The Minimum Inhibitory Concentration (MIC) and Minimum Bacteriosidal Concentration (MBC) of the PCFFF against Eschericia coli Extended Spectrum Beta Lactamase (ESBL) and Methycillin Resistant Staphylococcus aureus (MRSA) has also been done. Determination of the MM1 and MM2 PCFFF potential ratio to vancomycin against MRSA were also reported. It was found that, the PCFFF was unable to inhibit the growth of Mycobacterium tuberculosis, but the MM1 and MM2 PCFFF were able to inhibit the growth of ESBL and MRSA with MIC values of 25% and MBC 50% respectively, while the MM3 PCFFF was unable to inhibit ESBL at 100% concentration. The MM3 PCFFF showed inhibitory activity against MRSA at a concentration of 100%. The potential ratio of the MM1 and MM2 PCFFF to vancomycin standard solution (10, 5, and 2.5 ppm) against MRSA were 92.70% and 82.77% respectively.