Abstract
The antibiotics resistant micro-organism has increased in past three decades. The Methicillin Resistant Staphylococcus aureus (M.R.S.A) and Vancomycin resistant Enterococcus faecalis (VRE) is a problematic in the field of medical science. This organism tends to cause serious infection in the burnt patients and mortality. MRSA is a major nosocomial pathogen. Thereby our study aims to control these micro-organisms using various plants extracts. Four plant species Ageratum conyzoides, Phyllanthus emblica, Camellia sinensis and Mentha longifolia were collected and subjected to ethanolic extraction. The ethanolic extracts were tested against clinically isolated multidrug resistance Enterococcus faecalis and Staphylococcus aureus. The ethanolic plants extracts were prepared at variable concentrations for Staphylococcus aureus and Enterococcus faecalis. In view of MRSA against clinically isolated Staphylococcus aureus and Enterococcus faecalis the concentration based analysis showed the zone of inhibition against the selected micro-organism in which Camellia sinensis was found potent against Staphylococcus aureus and Mentha longifolia against Enterococcus faecalis. In future after the detailed study it can be used as a potent biochemical drugs to overcome the infections caused by these organisms.
Highlights
The emergence and spread of multidrug resistant (MDR) bacterial pathogens have substantially threatened the current antibacterial therapy [1]
The plant extracts were prepared in different concentrations ranging from 0.2 mg/ml to 2.0 mg/ml using four different plants, the best results were obtained by enlisted concentration of plantsAgeratum conyzoides (Family- Asteraceae) 0.42 mg/ml and 1.52 mg/ ml, Phayllanthus emblica (Family- Phyllanthacea) 0.75 mg/ml and 0.85 mg/ml, Camellia sinensis (Family- Theaceae) 0.85mg/ml and 0.70 mg/ ml and Mentha longifolia (Family- Lamiaceae) 1.32 mg/ml and 0.55 mg/ml for Methicillin resistant Staphylococcus aureus (MRSA) and Vancomycin Enterococcus faecalis (VRE), respectively
These extracts were tested for antimicrobial activity against gram positive bacteria Methicillin resistant Staphylococcus aureus (MRSA) isolated from burnt patients and gram positive bacteria Vancomycin Enterococcus faecalis (VRE) isolated from patients suffering from nosocomial infections
Summary
The emergence and spread of multidrug resistant (MDR) bacterial pathogens have substantially threatened the current antibacterial therapy [1]. There is a need to look for substances from other sources with proven antimicrobial activity This has led to the search for more effective antimicrobial agents among materials of plant origin, with the aim of discovering potentially useful active ingredients that can serve as source and template for the synthesis of new antimicrobial drugs [10,11]. Many plant species have been used by the indigenous people of India as traditional medicines, including as treatments for infectious diseases. Successful determination of such biologically active compounds from plant material is largely dependent on the type of solvent used in the extraction procedure.
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