Abstract
Methicillin-resistant Staphylococcus aureus (MRSA) has been emerging worldwide as one of the most important problems in communities and hospitals. Therefore, new agents are needed to treat acute oral infections from MRSA. In this study, antibacterial compounds from the roots of Atractylodes japonica (A. japonica) were isolated and characterized. The compounds were isolated from the root extracts using HPLC-piloted activity-guided fractionations. Four A. japonica compounds were isolated and identified as atractylenolide III (1), atractylenolide I (2), diacetylatractylodiol [(6E,12E)-tetradeca-6,12-diene-8,10-diyne-1,3-diol diacetate, TDEYA, 3). and (6E,12E)-tetradecadiene-8,10-diyne-1,3-diol (TDEA, 4), which was obtained by hydrolysis of TDEYA. The minimum inhibitory concentrations (MICs) was determined in the setting of clinical MRSA isolates. Compound 4 showed anti-MRSA activity with a MIC value of 4-32 μg/mL. The overall results provide promising baseline information for the potential use of the extract of A. japonica as well as some of the isolated compounds in the treatment of bacterial infections.
Highlights
Methicillin-resistant Staphylococcus aureus (MRSA) accounts for a large proportion of hospitalacquired infections and is considered a serious problem because of its multi-drug resistant properties.Currently, vancomycin and its analog teicoplanin are the most effective antibiotics for MRSA infections, but their clinical use often results in unexpected side effects and the development of vancomycin-resistant S. aureus infections
It was reported that A. japonica root extract protects osteoblastic MC3T3E1 cells against hydrogen peroxide-induced inhibition of osteoblastic differentiation [6]
Our ongoing efforts to find bioactive natural products have led us to study the antibacterial activity of A. japonica, which has been known to possess a variety of pharmacological properties against arthritis, bronchitis and respiratory infectious disease and to contain more than 50 phytochemicals, including atractylon and its derivatives [7,8], sesquiterpenoids
Summary
Methicillin-resistant Staphylococcus aureus (MRSA) accounts for a large proportion of hospitalacquired infections and is considered a serious problem because of its multi-drug resistant properties. The search for better drugs to combat MRSA infection is urgently needed. Atractylodes japonica is known to be effective for the control of pain and treatment of arthritis. It was reported that the Atractylodes family possesses anti-inflammatory activity [3], and modulates the intestinal immune system [4]. Atractylon, a major component, and its derivatives isolated from the rhizome were shown to have antihepatic properties [5]. The antibacterial effects of A. japonica on MRSA has not been evaluated. In the course of our ongoing project on the detection of bioactive compound from medicinal plants, the CHCl3-soluble extract of roots of A. japonica was found to exhibit distinctive antibacterial activity
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