Abstract
Advances in nanotechnology provide opportunities for the prevention and treatment of periodontal disease. While physicochemical properties of Ag containing nanoparticles (NPs) are known to influence the magnitude of their toxicity, it is thought that nanosilver can be made less toxic to eukaryotes by passivation of the NPs with a benign metal. Moreover, the addition of other noble metals to silver nanoparticles, in the alloy formulation, is known to alter the silver dissolution behavior. Thus, we synthesized glutathione capped Ag/Au alloy bimetallic nanoparticles (NPs) via the galvanic replacement reaction between maltose coated Ag NPs and chloroauric acid (HAuCl4) in 5% aqueous triblock F127 copolymer solution. We then compared the antibacterial activity of the Ag/Au NPs to pure Ag NPs on Porphyromonas gingivalis W83, a key pathogen in the development of periodontal disease. Only partially oxidized glutathione capped Ag and Ag/Au (Au:Ag≈0.2) NPs inhibited the planktonic growth of P. gingivalis W83. This effect was enhanced in the presence of hydrogen peroxide, which simulates the oxidative stress environment in the periodontal pocket during chronic inflammation.
Highlights
Advances in nanotechnology provide opportunities for the fabrication of silver containing nanoparticles (NPs) that can act as antimicrobial agents [1]
One of these “key pathogens” is Porphyromonas gingivalis, a black-pigmented gram-negative anaerobe. This anaerobe resides within the low oxygen environment of the periodontal pocket [19] and Journal of Nanomaterials is implicated in manipulating the host immune system and bringing about changes in the oral microbial community that contribute to chronic inflammation and tissue damage
Economic, and environmentally benign method for the synthesis of glutathione capped Ag/Au bimetallic NPs
Summary
Advances in nanotechnology provide opportunities for the fabrication of silver containing nanoparticles (NPs) that can act as antimicrobial agents [1]. Infection-induced periodontal disease is acknowledged to be polymicrobial in nature [15, 16] with the key pathogens, “the red complex,” being anaerobes [17, 18] One of these “key pathogens” is Porphyromonas gingivalis, a black-pigmented gram-negative anaerobe. This anaerobe resides within the low oxygen environment of the periodontal pocket [19] and Journal of Nanomaterials is implicated in manipulating the host immune system and bringing about changes in the oral microbial community that contribute to chronic inflammation and tissue damage [20]. Elevated levels of oxidative species are known to cause oxidative damage to tissues and is termed “oxidative stress”
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
