Antibacterial activity of liquid smoke from Anacardium Occidentale and Cocos nucifera shell

Abstract

Liquid smoke of Anacardium occidentale and Cocos nucifera shell has the ability as antibacterial. The study aimed to evaluate the inhibition of liquid smoke of A. occidentale and C. nucifera shell against Escherichia coli growth In Vitro. Parameters measured were phenolic, saponin, and tannin content, and pH value of liquid smoke,. The antibacterial activity test was carried out by well diffusion methods with 4 replications. Data were analyzed usinga completely randomized design. The results showed that the content of total phenol, saponins, and tannins was significantlyhigher (1.24%, 2.95%, and 0.48%) in C. nucifera liquid smoke (CONULIS) than in A. occidentale nutshell liquid smoke (ANOLIS) (0.71%, 1.36%, 0.17 %). The degree of acidity (pH) at ANOLIS is higher than pH of CONULIS. Antibacterial activity with a concentration of 10-100% showed that the inhibition (clear zone) of CONULIS was higher (10.43-22.88 mm) than ANOLIS (7.83-17.12) against E. coli. It can be concluded that C. nucifera shell liquid smoke (CONULIS) was better than A. occidentale liquid smoke (ANOLIS) to inhibit the growth of Escherichia coli In Vitro.