Abstract
The aim of the study was to investigate the leukocyte- and platelet-rich plasma (L-PRP) antimicrobial activity. The studied sample comprised 20 healthy males. The L-PRP gel, liquid L-PRP, and thrombin samples were tested in vitro for their antibacterial properties against selected bacterial strains using the Kirby-Bauer disc diffusion method. Two types of thrombin were used (autologous and bovine). Zones of inhibition produced by L-PRP ranged between 6 and 18 mm in diameter. L-PRP inhibited the growth of Staphylococcus aureus (MRSA and MSSA strains) and was also active against Enterococcus faecalis and Pseudomonas aeruginosa. There was no activity against Escherichia coli and Klebsiella pneumoniae. The statistically significant increase of L-PRP antimicrobial effect was noted with the use of major volume of thrombin as an activator. Additionally, in groups where a bovine thrombin mixture was added to L-PRP the zones of inhibition concerning MRSA, Enterococcus faecalis, and Pseudomonas aeruginosa were larger than in the groups with autologous thrombin. Based on the conducted studies, it can be determined that L-PRP can evoke in vitro antimicrobial effects and might be used to treat selected infections in the clinical field. The major volume of thrombin as an activator increases the strength of the L-PRP antimicrobial effect.
Highlights
Due to their numerous important properties, platelets started to be used in the contemporary medicine as a strategy to stimulate tissue healing, as an autologous cell and plasma fraction extracted from the peripheral blood [1]
Previous in vitro investigations showed that leukocyteand platelet-rich plasma (L-PRP) gel is active against selected bacterial strains [6,7,8]
Hematology analysis revealed that average total white blood cells count in the L-PRP amounted to 29.79±5.89 k/μl
Summary
Due to their numerous important properties, platelets started to be used in the contemporary medicine as a strategy to stimulate tissue healing, as an autologous cell and plasma fraction extracted from the peripheral blood [1]. Most authors believe that blood concentrate activation and the release of platelet-derived growth factors lead to the acceleration of the wound healing process [1,2,3]. Previous in vitro investigations showed that leukocyteand platelet-rich plasma (L-PRP) gel is active against selected bacterial strains [6,7,8]. Some authors reported clinical observations about a decreasing number of infections and the induction of healing processes after L-PRP use in orthopedics [9], trauma [10], maxillofacial [11], and cardiac surgery [12]
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