Antibacterial Activity of Indonesian Sponge Associated Fungi Against Clinical Pathogenic Multidrug Resistant Bacteria

Mada Triandala Sibero ,Agus Sabdono ,Agus Trianto ,Ocky Karna Radjasa ,Desy Wulan Triningsih ,Inggrid Debora Hutagaol

doi:10.7324/japs.2018.8214

Abstract

Exploration of new source of antibiotics to combat multidrug-resistant bacteria is urgently needed. Indonesia as archipelago country has a wide variety of marine organisms with potential as source of new antibacterial compounds against MDRO. Aims of the study were to isolate sponge-associated fungi from sponge Cinachyrella sp. collected from Pandang Island, North Sumatera, Indonesia, to screen potential fungi against clinical pathogenic MDR bacteria, to identify the potential fungi; and to determine the best cultivation time for antibacterial production. Nine spongeassociated fungi were successfully isolated. Result of agar plug method showed fungus PDSP 5.7 was the most potential candidate which inhibited ESBL Escherichia coli, Salmonella enterica ser. Typhi, MRSA, and Staphylococcus haemolyticus strain MDR. This fungus had 100% similarity to Trichoderma reesei. In malt extract broth, T. reesei PDSP 5.7 had stationary phase from day 12 to day 18. In addition, the widest antibacterial was performed by extract from day 15. Furthermore, fungal extract showed best antibacterial activity against S. enterica ser. Typhi strain MDR with inhibition value of 14.72 ± 0.07 mm2.

Highlights

The massive number of multidrug-resistant organisms (MDRO) infections nowday is caused by the irrational use of antibiotic in several decades ago (Alanis, 2005; de Simone et al, 2016; Cansizoglu and Toprak, 2017)
Indonesia as archipelago country has a wide variety of marine organisms with potential as source of new antibacterial compounds against MDRO
The clinical pathogenic MDR bacteria used in this study were clinical collections from Dr Kariadi General Hospital Medical Center, Semarang, Central Java, Indonesia

Summary

INTRODUCTION

The massive number of multidrug-resistant organisms (MDRO) infections nowday is caused by the irrational use of antibiotic in several decades ago (Alanis, 2005; de Simone et al, 2016; Cansizoglu and Toprak, 2017) Several pathogenic bacteria such as extended-spectrum beta-lactamase (ESBL) E. coli, methicillin-resistant S. aureus (MRSA), Staphylococcus haemolyticus, and Salmonella enterica ser. Several discoveries were successfully isolate new compounds from sponge-associated fungi with biological activities (Zin et al, 2016; Noinart et al, 2017). Fungus Pestalotiopsis heterocornis which isolated from sponge Phakellia fusca was reported to produce isocumarins 1-3 These compounds had antibacterial activity against S. aureus and B. subtilis with MIC values ranging from 25 to 100 μg/mL (Lei et al 2017). Aims of the study were to isolate sponge-associated fungi from sponge Cinachyrella sp. collected from Pandang Island, North Sumatera, to screen potential fungi against clinical pathogenic MDR bacteria, to identify the potential fungi, and to determine the best cultivation time for antibacterial production

MATERIAL AND METHODS

AND DISCUSSION

CONCLUSION