ANTIBACTERIAL ACTIVITY OF DROSERA ROTUNDIFOLIA L. AGAINST GRAM-POSITIVE AND GRAM-NEGATIVE BACTERIA

Dominika Ďurechová,Jana Petrová,Lukã¡Å¡ Hleba,Iwona Kata,Miroslava Kačániová,Margarita Terentjeva

doi:10.15414/jmbfs.2016.5.special1.20-22

Abstract

The medicinal use of genus Drosera, as an important antitussive for different respiratory diseases, has been known for centuries. Many of extracts from carnivorous plants exhibit various antibacterial and antifungal activities. Naphthoquinones containing extracts from Drosera have antiviral, antibacterial, antifungal, aphrodisiac, antispasmodic, antileprosy, antisclerotic and anticancer properties. The aim of the present study was to detect antibacterial activity of Drosera rotundifolia against Gram-positive and Gram-negative bacteria by the testing of MIC. For the study six strains of microorganisms were selected and there were Gram-positive bacteria -Bacillus thuringiensis (CCM 19T), Clostridium perfringens (CCM 4991), and Listeria monocytogenes (CCM 4699), as well as and Gram-negative bacteria - Escherichia coli (CCM 3988), Salmonella enterica subsp. enterica (CCM 3807) and Yersinia enterocolitica (CCM 5671). Plant extracts were isolated from three plants of Drosera rotundifolia L. (S1, S2 and S3) in different time range. The most effective extract with MIC50 value of 17.07 μg.ml-1 was S3, while forMIC90 of 19.05 μg.ml-1 were extracts S2 and S3 exhibiting antimicrobial activity against Bacillus thuringiensis, Clostridium perfringens and Listeria monocytogenes. Extracts S1, S2 showed MIC50 value 25.53 μg.ml-1for all the microorganism tested, but S3 extract revealed the same antimicrobial activity against Yersinia enterocolitica, Salmonella enterica subsp. enterica and Escherichia coli. Extract S1 has MIC90 value of 27.14 μg.ml-1 against all the microorganism tested, but S2 and S3 shared the same MIC90 for Yersinia enterocolitica, Salmonella enterica subsp. enterica and Escherichia coli.

Highlights

Plants have several ways of taking up nutrients; among them one of the highly specialized way is carnivory
Medicinal use of Drosera is convenient due to the simplicity of its cultivation in vitro. Drosera extracts owe their antimicrobial properties to secondary metabolites
Naphthoquinones, mainly plumbagin are the main active compounds produced by D. binata tissues

Summary

Introduction

Plants have several ways of taking up nutrients; among them one of the highly specialized way is carnivory. Carnivorous plants capture and utilize nutrients of prey, which consists mostly from insects (Darwin, 1875; Juniper et al, 1989). These plants occur in areas such as wetlands, alpine mountain peaks, vulcanic platform. The naphthoquinones, and specially plumbagin, inhibits a development of parasitic nematodes and insects (Collantes et al, 2014). The present study was focused on Drosera rotundifolia of genus Drosera, which might be a pharmacologically important plant for its antimicrobial activity. The aim of this study was to evaluate the antibacterial activity of Drosera plant extracts by the detection of the minimal inhibitory concentration (MIC). Antimicrobial activity of six bacteria: Gram-positive _ Bacillus thuringiensis, Clostridium perfringens, Listeria monocytogenes and Gram-negative bacteria Yersinia enterocolitica, Salmonella enterica subsp. enterica and Escherichia coli were evaluated

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