Abstract
Objective: To investigate the in vitro antibacterial activity of a naturally occurring polyphenol chlorogenic acid (CGA) and compares it with formulated chlorogenic acid phytovesicles against 4 different bacterial strains; two gram positive [Staphylococcous aureus and Bacillus subtilis] and two gram negative strains [Klebsiella pneumonia and Escherichia coli]. Methods: CGA phytovesicles were developed and optimized using central composite design to improvise CGA’s physicochemical properties. Bactericidal activity was evaluated using agar diffusion, minimum inhibitory concentration (MIC) and time kill assay. The effect of pH and temperature on the antimicrobial activity was determined. Results: The optimized CGA phytovesicles showed entrapment of 96.89% with 30 times better lipophilic solubility than the plain drug. The inhibition zone sizes for CGA phytovesicle ranged from 17-25 mm as compared to 15-20 mm of plain CGA while the MIC values ranged 200-250 µg/ml as compared to 500-550 µg/ml of plain CGA. CGA phytovesicles exhibited a strong bactericidal effect at MIC with a log reduction in the range of 0.90-2.04 in Colony forming units (CFUs) at 24h for different strains as compared to 1.38-2.17 of plain CGA. Furthermore, the antibacterial effect was found to augment with increasing temperature but decreased with alkaline pH. Conclusion: Results strongly supports the hypothesis of potential use of CGA phytovesicles as a mode of drug delivery for its antibacterial use against different resistant bacteria.
Highlights
Phytochemicals are the bioactive plant compounds present in functional foods like fruits, vegetables, grains etc
Natural antimicrobials don’t target a particular reaction rather act on various groups of biomolecules in a pathogen there is no occurrence of resistance which makes them more alluring candidate [3]
chlorogenic acid (CGA) phytovesicles were optimized by determining the effect of three different variables-drug-phospholipid ratio (X1), reaction time (X2) and reaction temperature (X3) which greatly influenced partition coefficient (Y1) and entrapment efficiency (Y2) of CGA phytovesicles
Summary
Phytochemicals are the bioactive plant compounds present in functional foods like fruits, vegetables, grains etc. These bioactive compounds not just promote good health but play a major role in prevention of chronic diseases [1]. Natural antimicrobials don’t target a particular reaction rather act on various groups of biomolecules in a pathogen there is no occurrence of resistance which makes them more alluring candidate [3] These bioactive compounds or secondary metabolites are limited in their efficacy because of poor absorption with limited solubility in lipophilic phases and instability at different physiological pH values [4]
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
