Abstract
In this study, Clove Oil (CO) was encapsulated into Halloysite Nanotubes (HNT) and the developed nanocapsule (CO-HNT) were further combined with chitosan for the formulation of active food packaging film. CO encapsulation efficiency into HNT was determined to be 7.54 % using thermogravimetric analysis. The developed nanocomposite film (CO-HNT/chitosan) was characterized by several instruments viz. SEM, DSC, FTIR and UV–visible Spectrophotometer. The antioxidant activity of nanocomposite film was determined by DPPH assay and the maximum antioxidant activity (74 %) was found for the concentration of film extract of 100 µl/ ml of DPPH. In vitro antimicrobial studies of the developed nanocomposites showed effective antibacterial activity at about 99 % against Bacillus mojavensis and Escherichia coli at concentrations of 3 mg/ml and 4 mg/ml respectively. In addition, CO-HNT/chitosan nanocomposites film exhibited a zone of inhibition against these bacterial strains and blocked the UV light up to 99 % without sacrificing the transparency of the films. Hence, this nanocomposite film could find applications in the area of food packaging.
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