Abstract
The complex of chitosan and hyaluronic acid was treated with enzyme to obtain chitosan oligosaccharide designated to COS hereafter. The degree of decomposition was confirmed by reducing sugar quantitative method, and the molecular weight size was confirmed by Matrix Assisted Laser Desorption Ionization Time-of-Flight (MALDI-TOF) mass spectrometry analysis. Based on the fact that the substrate specificity of the enzyme is highly dependent on temperature, substrate concentration, and pH, etc, the COS obtained by treating a mixture of chitosan and hyaluronic acid, which physically mixes well, under the non-optimal reaction conditions of the chitosanase. Accordingly, it exhibited very significant antibacterial activity against Meticillin Resistant Stapylococcs aureus (MRSA). As a method for controlling molecular weight of COS, it was intended to propose a new enzyme usage as a study to identify the characteristics of the hydrolysates according to the reaction delay and enzyme activity through complex formation.
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