Abstract
Bacterial cellulose-based edible films have been known as natural material for packaging containing carboxymethyl cellulose (CMC) and glycerol. Edible film is also known as an external food protection since it contains antibacterial substance. Incorporating antimicrobial compounds with essential oil (EO) from Citrus spp such as lime, lemon and sweet orange into edible films provides a novel approach to improve the safety, shelf-life of foods or fruits as well as physical properties of the edible film. The present study was aimed to investigate the antibacterial activity of edible film incorporated with EO. Antibacterial activity performance was carried out using broth and dilution agar method to determine EO role to inhibit the growth of pathogen bacteria such as Escherichia coli and Staphylococcus aureus. The results showed that edible film containing 2% lime, lemon and sweet orange performed moderate antibacterial activity with minimum inhibition concentrations (MICs) of about 100, 250 and 225 mg/mL, respectively.
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