Antibacterial activity of aquatic actinomycete,Actinoplanes digitatisDnj-2 (MN567713)

Ali Mohammed Abdullah Bawazir ,Manjula Shantaram,M Jayashankar

doi:10.51248/.v41i1.535

Abstract

Introduction and Aim: There are centers for antibiotics in the nature to treat disease by looking for different sources, for example, actinomycetes, are approximately two-thirds of naturally occurring antibiotics, having much of medical importance, have been isolated from actinomycetes.The aim of the present study was to isolate and to identify the actinomycetes having antagonistic activity.  Materials and Methods: An actinomycetes strain isolated from dam soil sample collected from Alnajl dam of Hadhramout Yemen, showed antibacterial activity against selected microbial pathogens. The nutritional requirements and cultural conditions for maximal growth to produce secondary metabolites have been optimized under shake flask conditions. The growth and production of secondary metabolites were maximal with the use of SCA medium supplemented at pH 7.0, and incubation temperature and time of 37?and 20 days respectively.  Results:Based on morphological, biochemical, physiological and phylogenetic characterization, the strain was identified as Actinoplanes digitatisDnj-2 (MN567713). The ethyl acetate extract (1g/mL) obtained from the isolate showed significant antibacterial activity against Gram-negative bacteria- Escherichiacoli (21cm) and Pseudomonas aeruginosa (29cm) and Gram-positive bacteria- Bacillus subtitles(31cm) and Staphylococcus aureus (22cm) when compared with penicillin G 10 units.  Conclusion:In conclusion, the isolated strain has broad spectrum of antagonistic activity against Gram-positive and Gram-negativebacteria.

Highlights

Introduction and AimThere are centers for antibiotics in the nature to treat disease by looking for different sources, for example, actinomycetes, are approximately two-thirds of naturally occurring antibiotics, having much of medical importance, have been isolated from actinomycetes
At present, twothirds of natural antibiotics are obtained from actinomycetes and they serve as substitutional sources of biologically active substances [2]
The genus Actinoplanes was classified under the group Actinoplanetes in Bergey's Manual of Determinative Bacteriology [5]. Genera in this group were distinguished from each other based on the shape of the sporangium, number, shape, and arrangement of spores in the sporangium, motility, and flagellation of the spores and other such characters

Summary

Introduction

Introduction and AimThere are centers for antibiotics in the nature to treat disease by looking for different sources, for example, actinomycetes, are approximately two-thirds of naturally occurring antibiotics, having much of medical importance, have been isolated from actinomycetes. At present, twothirds of natural antibiotics are obtained from actinomycetes and they serve as substitutional sources of biologically active substances [2]. The genus Actinoplanes was classified under the group Actinoplanetes in Bergey's Manual of Determinative Bacteriology [5] Genera in this group were distinguished from each other based on the shape of the sporangium, number, shape, and arrangement of spores in the sporangium, motility, and flagellation of the spores and other such characters. Identification of isolate to the species level was done based on the morphology of sporangium, presence or absence of aerial mycelium and assimilation of carbon sources as well as a molecular genome. Species belonging to this genus were identified as Actinoplanes digitatis. At the time of writing report, the genus involves 45 species with validly published names, including as those of late described Actinoplanes lichenis [6], Actinoplanes subglobosus [7], Actinoplanes subg lobosus [8], Actinoplan-es ramoplaninifer [9]

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