Antibacterial Activity of Antibiotic-Impregnated Bone Cement Based Coatings Against Microorganisms with Different Antibiotic Resistance Levels

D V Tapalski,A I Kozlova,A Sitnik,P A Volotovski

doi:10.21823/2311-2905-2018-24-4-105-110

Abstract

Purpose — to evaluate the presence and duration of antibiotic activity of antibiotic-impregnated bone cement based coatings samples against antibiotic-sensitive and antibiotic-resistant microorganisms.Material and Methods. Bone cement based coatings impregnated with antibiotics (gentamycin, vancomycin, colistin, meropenem, fosfomycin) are formed on titanium (Ti) plates. A plate rinse was carried out; antibiotic concentrations in the rinsed solutions were estimated by a serial broth microdilution method. Antibacterial activity of the control and rinsed samples against the antibiotic-sensitive and multiple-antibiotic-resistant Staphylococcus aureus and Pseudomonas aeruginosa strains was estimated by a bilayer agar method.Results. The meropenem and fosfomycin concentrations in the rinsed solutions obtained at a one-fold (16 μg/ml for both antibiotics) and two-fold treatment (2 μg/ml for meropenem and 8 μg/ml for fosfomycin) were sufficient to suppress the growth of the control strains. One-fold rinse of samples with colistin eliminated their antibacterial activity completely. The marked activity of the samples with meropenem and fosfomycin persisted against the antibiotic-sensitive P. aeruginosa ATCC 27853 strain after 2 rinse cycles; single-rinsed samples with fosfomycin also maintained the activity against the extensively antibioticresistant P. aeruginosa BP-150 strain. Vancomycin-containing samples possessed the sufficient antibacterial activity against both methicillin-sensitive (MSSA) and methicillin-resistant (MRSA) S. aureus strains; two-fold rinse of the samples eliminated their bactericidal properties.Conclusion. Bone cement based coatings impregnated with fosfomycin and meropenem possess the most marked and long-lasting antibacterial activity, manifested mainly against the antibiotic-sensitive strains.

Highlights

Антибактериальная активность покрытий из костного цемента и антибиотиков в отношении P. aeruginosa ATCC 27853 и клинического изолята P. aeruginosa БП-150, двухслойный агаровый метод: a — гентамицин (0,5%); b — колистин (0,6%); c — меропенем (5%); d — фосфомицин (5%)
Эффективное использование в составе покрытий колистина возможно только при увеличении его концентрации в составе костного цемента, что требует проведения дополнительных исследований in vitro

Summary

Introduction

Цель исследовани — оценить наличие и продолжительность антибактериальной активности образцов покрытий на основе костного цемента, импрегнированного антибиотиками, в отношении антибиотикочувствительных и антибиотикорезистентных микроорганизмов. Оценку антибактериальной активности нанесенного на титановые пластины костного цемента (для контрольных и отмытых пластин) проводили двухслойным агаровым методом [2]. Значения МПК антибиотиков, используемых для включения в состав костного цемента, для тест-культур представлены в таблице 1.

Results

Conclusion