Abstract

The Cuminum Cyminum (cumin) oil was extracted from cumin seeds by distillation process. The extracted cumin oil was used to assess its effectiveness as antibacterial that through testing on six types of bacteria; two of them were bacteria gram-negative (E. coli and S. typhi) and the remainders were bacteria gram-positive (Proteus Vulgaris, Klebsiella Pneumonae, Enterococcus Feacalis and Staphylococcus Aureus). Four concentrations (12.5%, 25%, 50% and 100%) of cumin oil were used for screening fulfillment by using the cup-plate agar diffusion method and gentamicin (10µg) as the positive control. According to different concentration the inhibition area, minimum inhibition zones diameters (MIZD) in mm and the relative percentage inhibition of the test with respect to positive control were calculated. The results showed that all tested concentrations of cumin oil showed antibacterial activity against gram positive and gram negative bacteria.

Highlights

  • Cuminum Cyminum L is an herbaceous and medicinal crop and one of the oldest and popular seed spice worldwide after black pepper [1]

  • The inhibition area and minimum inhibition zones diameters (MIZD) and percentage were calculated according to different concentration (12.5%, 25%, 50% and 100%) and represented in tables 1 & 2 and figure 1 below

  • The results showed that all tested concentrations of cumin oil showed antibacterial activity against gram positive and gram negative bacteria, only Enterococcus fecalis was resistance at the concentrations 12.5% and 50%

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Summary

Introduction

Cuminum Cyminum L is an herbaceous and medicinal crop and one of the oldest and popular seed spice worldwide after black pepper [1]. The term ‘spice’ originated from the Latin word ‘species’, meaning of specific kind. The two terms may be used for the same plants in which the fresh leaves are used as herbs, while other dried parts are used as spices [2]. Cumin was originally cultivated in Iran, where it is one of the most important export crops, and Mediterranean region but today it is grow in Uzbekistan, Tajikistan, Turkey, Morocco, Egypt, India, Syria, Mexico, Bulgaria, Cyprus and Chile, where India is the largest producer and consumer of cumin seed in the world [4]

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