Abstract
Antibacterial Activity of Actinobacteria Isolated from Mangroves of Andaman and Nicobar Islands, India
Highlights
Mangroves are coastal wetland forests mainly found at the intertidal zone of estuaries, back waters, deltas, creeks, lagoons and mudflats of tropical latitudes
While in Carbyn’s Cove, total heterotrophic bacterial count (THBC) ranged between 20 + 2.16 X 106 in the middle zone and 42 + 1.7 X 106 in the distal zone
Identification of Actinobacteria 23 colonies of actinobacteria were isolated from the sediment samples during the present study
Summary
Mangroves are coastal wetland forests mainly found at the intertidal zone of estuaries, back waters, deltas, creeks, lagoons and mudflats of tropical latitudes. The mangrove environment is a potent source for the isolation of antibiotic producing Actinobacteria, the. The Actinobacteria from the sediments of two mangrove environments (Burmanallah and Carbyn’s Cove) of South Andaman region was carried out for their diversity and antibacterial activity of the Actinobacteria extract against six pathogens. 1 ml was inoculated into antibiotic incorporated Amphotericin (10 μL) and Nystatin (50 μL) starch casein agar medium by pour plate method and incubated at 28oC for 7 to 9 days (Sahoo, 2009; Sivakumar et al, 2005). The isolates were inoculated in Marine Actinobacteria growth (MAG) broth and incubated for 4-5 days and observed for aerial mycelium under 100x light microscope. Utilization of carbohydrates, salt tolerance 0 to 20% (w/v) NaCl and temperature tolerance along with urease activity, oxidase test, catalase test, protease, lipase, amylase, chitinase and cellulase activity were tested (El-Habib and Magda, 2012; Gopalakrishnan et al, 2014)
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