Antibacterial Activity by Diffusion and Dilution Methods of Liquid Smoke Cocos nucifera L.

Abstract

Introduction: Liquid smoke is a product that obtained industrially from coconut shell through pyrolysis process at 400°C for 2,5 hours and redestillation to obtain grade 1 liquid smoke. Coconut shell liquid smoke contains chemical compounds, namely phenol, which has been researched to act as a disinfectant that has broad spectrum antibacterial activity against gram positive and gram negative bacteria. Method: This study aims to determine the potential of cocos nucifera L. liquid smoke as an antibacterial against Staphylococcus aureus and Methicillin-resistant staphylococcus aureus (MRSA) bacteria using diffusion and dilution methods. The concentrations used were 100%, 80%, 60%, 40%, 35%, 30%, 15%, 10%, 7%, 5%. As positive control was used Tetracycline 25 ppm. Results: Liquid smoke has the potential to inhibit the growth of both bacteria. The effective concentration to inhibit the growth of S. aureus at 40% concentration and MRSA at 80% concentration. The MIC value of liquid smoke against S. aureus is 0,78%, while on MRSA 3,12%. The MBC of S. aureus and MRSA bacteria at concentrations 1,56% and 6,25%. Staphylococcus aureus has higher sensitivity to Cocos nucifera L. grade 1 liquid smoke than MRSA bacteria. Conclusion: Cocos nucifera L. liquid smoke has antibacterial activity against Staphylococcus aureus and MRSA at effective concentrations of 40% and 80%. The MIC value of S. aureus is 0,78%, while MRSA is 3,12%. The MBC of S. aureus and MRSA bacteria at concentrations 1,56% and 6,25%. Keywords: antibacterial, liquid smoke, phenol, Cocos nucifera L.