Abstract
The aim of this research is to investigate the antibacterial activity and identify the phytochemical constituents of Mangifera indica leafs on Pseudomonas aeruginosa and Staphylococcus aureus using disc diffusion method. The sample was collected fresh from the premises of Bioresources Development Center (BIODEC), Katsina, Katsina State and was dried and pounded into powder. The powdered leaves were extracted using ethanol and aqueous solvents. Various concentrations ranging from 500mg to 62.5mg were prepared. Test isolates were obtained from the Microbiology laboratory, Umaru Musa Yar’adua University Katsina (UMYUK) and were further authenticated using Gram staining and biochemical test. The bacterial inoculums were standardized to McFarland scale 0.5. Zones of inhibition were read after 24 hours at 370C. The results of the antibacterial study revealed that the ethanolic leaves extracts at 500mg/ml had effect on P. aeruginosa and S. aureus with zones of inhibition of 12mm and 6mm respectively. The results of the phytochemical screening revealed the presence of flavonoids, tannins, saponins, alkaloids and phenols where only alkaloids was found to be absent in the aqueous extract. There is no significant difference between the solvents and various concentrations used base on t-test data analysis.
Highlights
OF THE STUDYThe emergence of multi-resistant bacteria to antimicrobial drugs has increased the need for new antibiotics or modifications of older antibiotics (Tollefson and Miller 2000)
One way to prevent antibiotic resistance of pathogenic species is by using new compounds that are not based on existing synthetic antimicrobial agent (Mathur et al, 2011)
Crude antibacterial activity for both ethanol and aqueous mango leaves extract in this study reveals that all the test bacteria were susceptible to both the extracts at 500mg/ml, 250mg/ml, and 125 mg/ml concentrations on Pseudomonas aeruginosa and Staphylococcus aureus but with the exception of 62.5mg concentration of mango leave aqueous extract on Staphylococcus aureus which shows resistance
Summary
BACKGROUND OF THE STUDYThe emergence of multi-resistant bacteria to antimicrobial drugs has increased the need for new antibiotics or modifications of older antibiotics (Tollefson and Miller 2000). Reservoir of effective chemotherapeutics from plant families provides valuable sources of natural antimicrobials. Cheaper and safe alternative source of drugs in this contemporary period are mostly from plant bio-resources. Cases of overdose or self-poisoning through the use of medicinal plants have been increasing. After following up on ethno-medicinal use, an estimated 74% of pharmacologically active plant derived components were discovered. Mushore and Matuvhunye (2013) stated that more than 25% of modern medicines are thought to have descended from plants whilst others are synthetic analogues built on prototype compounds isolated from plants. The richest bio-resource of drugs of modern medicine, folk medicine and chemical entities for synthetic drugs nowadays are from medicinal plant sources
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
