Abstract

Ethanolic extract of the leaves of Ocimum gratissimum was screened for its phytochemical and antibacterial properties on Listeria monocytogenes at varying concentrations. The Agar gel diffusion method was used to assay for the antibacterial properties on the test isolate. The results showed that the ethanolic extracts at different concentrations inhibited the growth of Listeria monocytogenes. The concentration of 250mg/ml inhibited the isolate with highest diameter zone of inhibition of 25mm. The extracts inhibited the growth of the bacterial isolate in a concentration dependent manner with MICs of 9.25mg/ml, while MBCs had 2.15mg/ml. Phytochemical analysis of the leaf extracts revealed the presence of antimicrobial active agents such as alkaloids, cardiac glycosides, flavonoids, glycosides, resins, steroidal terpens, and tannins. These established a good support to the use of this plant in herbal medcine and as base for the development of new drugs and phytomedicine.

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