Acta Aquatica Turcica
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Antibacterial Activity and Characterization of Water-Soluble Chitosan Compounds Produced from Enzymatic Deacetylation

Publication Date Jun 20, 2022

Abstract

In this research, it was aimed to investigate the production of water-soluble chitosan from the enzymatic deacetylation of chitin by Bacillus sp. (B. cereus (BC) and B. thuringiensis (BT)) strains. Characteristic properties involving molecular weight, degree of deacetylation, and antibacterial activity of chitosan samples were identified. The degree of deacetylation of BC and BT chitosan samples obtained at 393 and 213 ppm amounts was reported as 80.54 and 86.07% by IR spectrum. As well as the degree of deacetylation, molecular weights of samples showed a significant effect on antibacterial activity were 48.27 and 48.46 Da, respectively. Among the tested bacteria, the highest inhibitory effect was recorded to P. aeruginosa and S. epidermis for both chitosan samples. On the other hand, no antibacterial effect on S. aureus, VRE, S. epidermidis, Klebsiella sp., and Salmonella spp were observed. Our results were indicated a simple and cost-efficient method for the production of chitosan polymers showing antibacterial activity.

Concepts

Degree Of Deacetylation Chitosan Samples Antibacterial Activity Production Of Chitosan Enzymatic Deacetylation Highest Inhibitory Effect B. Thuringiensis B. Cereus Antibacterial Effect Production Of Polymers

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