ANTIBACTERIAL ACTIVITIES TEST OF RED GINGER (Zingiber officinale var. rubrum) ETHANOL EXTRACT ON STAPHYLOCOCCUS EPIDERMIDIS

Abstract

Background: Antibiotic is a drug often used against infections caused by bacteria; however, irrational use of antibiotics can cause multidrug resistance (MDR). Staphylococcus epidermidis is a bacteria that has developed an MDR characteristic against antibiotics. Staphylococcus epidermidis is a normal opportunistic flora. Said bacteria can produce a biofilm that enables the bacteria to adhere to prosthetic devices and enter the bloodstream. Red ginger (Zingiber officinale var. rubrum) is a medicinal plant considered antibacterial for its secondary metabolites. Purpose: This study aims to determine the antibacterial effect of red ginger (Zingiber officinale var. rubrum) ethanol extract against Streptococcus epidermidis using a non-equivalent control group design study. Method: Red ginger (Zingiber officinale var. rubrum) ethanol extract was made with the maceration method. This study uses a microdilution test on 96 well plates. The absorbance value was read using a spectrophotometer to determine the Minimum Inhibitory Concentration (MIC) value, and streaking agar was used to find the Minimum Bactericidal Concentration (MBC) value. Result: The result shows that the red ginger (Zingiber officinale var. rubrum) ethanol extract at a concentration of 62,5 μg/mL inhibits 75% of bacteria growth, at 125 μg/mL inhibits 60% of bacteria growth, at 250 μg/mL inhibits 45% of bacteria growth, at 500μg/mL inhibits 65% of bacteria growth and at 1.000μg/mL inhibits 50% of bacteria growth. The streaking agar test result shows bacteria growth on every concentration. Conclusion: Red ginger (Zingiber officinale var. rubrum) ethanol extract in the concentration range of 62,5-1.000 μg/mL have no MIC and MBC value against Staphylococcus epidermidis.