Abstract
The addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory factor to antibiotic resistance, thus an alternative to antibiotics is needed in animal feed additives. To determine the antimicrobial activity of cow's intestinal Lactic acid bacteria (LAB) against enteric commensals. Escherichia coli, Klebsiella species (spp) and LAB were isolated from thirty different cow faecal samples and the LAB identified by partial sequencing of 16S rRNA. The antimicrobial activity of the LAB was determined against the test Escherichia coli and Klebsiella spp. Five species of LAB were isolated from thirty cow faecal samples and identified as Enterococcus hirae (8), Enterococcus durans (6), Enterococcus faecium (1), Enterococcus faecalis (1) and Weissella confusa (1). Viable cells and cell free supernatant (CFS) of the LAB were able to inhibit the growth of the test organisms with the largest zone of inhibition by the viable cells being 26mm against Escherichia coli CB6 produced by Enterococcus hirae CO6A while Weissella confusa CO29M and Enterococcus hirae CO2A produced the largest zones of inhibition (26mm) against Klebsiella CB2. This study shows that LAB from cow faeces possess considerable antimicrobial activity against resistant Escherichia coli from the same environment.
Highlights
Livestock sustain the livelihood of millions of people in the world in both developing and developed countries[1]
Seventeen lactic acid bacteria were isolated from thirty faecal samples collected from different healthy cows, for the purpose of determination of their antagonistic activities against Escherichia coli and Klebsiella spp isolated from the same environment
The colony forming unit per gram of the presumed Lactic acid bacteria (LAB) was carried out on the faecal samples with the level of lactic acid bacteria in cow faeces ranging from 3.8 x107 to 1.0 x 108 cfu/g has shown in table 1
Summary
Livestock sustain the livelihood of millions of people in the world in both developing and developed countries[1]. In 1946 experiments showed that low, sub therapeutic levels of antibiotics could increase feed efficiency and growth in food animals, and the addition of various antibiotics to feed for livestock was initiated[3]. The addition of sub therapeutic doses of antibiotics to cattle feed for growth promotion is a contributory factor to antibiotic resistance, an alternative to antibiotics is needed in animal feed additives. The antimicrobial activity of the LAB was determined against the test Escherichia coli and Klebsiella spp. Conclusion: This study shows that LAB from cow faeces possess considerable antimicrobial activity against resistant Escherichia coli from the same environment. Antibacterial activities of lactic acid bacteria isolated from cow faeces against potential enteric pathogens.
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