Abstract
Aims: In the present investigation, To synthesis, characterization and antibacterial activity of Calcareous/TiO2 nanocomposites toward Bacillus, Proteus vulgaris, Micrococcus luteus, Staphylococcus aureus and Escherichia coli.
 Methodology: The resulting Calcareous/TiO2 nanocomposite was synthesized by hydrothermal method. The synthesized nanocomposites were characterized by using XRD, SEM with EDAX, AFM, TEM and UV-Vis absorption spectroscopy. The antibacterial activities of the Calcareous/TiO2 nanocomposite species were checked by using agar well diffusion method.
 Results: The XRD pattern is showed that the diffraction peaks appear in the pattern corresponding to the anatase phase of TiO2. UV-Visible spectrum showed that the blue shifted when compared with bulk TiO2 (3.2 eV). The blue shift might be caused by nanosize effect and structural defect of nanomaterials. AFM image shows the morphology of Calcareous/TiO2 nanocomposites forming sharp particles on the surface. TEM image showed that the particles exhibit a relatively uniform particle size distribution. The average size of the nanocomposites estimated from the TEM image is around 50nm.
 Conclusion: In conclusion, the Calcareous/TiO2 nanocomposites have been successfully synthesized. The prepared nanocomposites were characterized using various analytical tools like XRD, SEM with EDAX, AFM, TEM, UV-Vis absorption spectrum.
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