Antiallergic Effects of Phenolic Compounds Isolated From Stellera chamaejasme on RBL-2H3 Cells

Beom-Geun Jo,Su-Nam Kim,Jonghwan Jegal,Min Hye Yang,Sim-Kyu Bong

doi:10.1177/1934578x20942352

Beom-Geun Jo, Su-Nam Kim + Show 3 more

Open Access

https://doi.org/10.1177/1934578x20942352

Copy DOI

Abstract

The current study was undertaken to isolate and identify anti-inflammatory and antiallergic active compounds in the ethyl acetate and butanol fractions of an ethanolic extract of the aerial parts of Stellera chamaejasme (SCAE). A new maltol glucoside, maltol 3-(6″-(2-( E)-butenoyl)-glucoside) and benzoic acid, daphnoretin (a coumarin), 6 flavonoids (apigenin, genkwanin, taxifolin, apigenin 7- O-glucoside, luteolin 7- O-sambubioside, genkwanin 5- O-glucoside), and 2 lignans (matairesinoside and lariciresinol) were isolated from SCAE by chromatographic separation. Their chemical structures were elucidated by analyzing spectroscopic data, including 1-dimensional and 2-dimensional nuclear magnetic resonance (Fig1). Maltol 3-(6″-(2-( E)-butenoyl)-glucoside) significantly inhibited β-hexosaminidase release by 85.5% in immunoglobulin E and dinitrophenyl/bovine serum albumin (DNP/BSA)-induced RBL-2H3 cells. Benzoic acid from S. chamaejasme inhibited β-hexosaminidase release (by 80.2%) in IgE and DNP/BSA-induced RBL-2H3 cells and interleukin-4 messenger ribonucleic acid expression (by 21.9% inhibition) in propidium iodide-induced RBL-2H3 cells.

Highlights

The current study was undertaken to isolate and identify anti-inflammatory and antiallergic active compounds in the ethyl acetate and butanol fractions of an ethanolic extract of the aerial parts of Stellera chamaejasme (SCAE)
immunoglobulin E (IgE)-m ediated allergies are classified as Type I hypersensitivities and include allergic conjunctivitis, allergic asthma, allergic rhinitis, and atopic dermatitis.[2,3]
Ten other compounds were identified in SCAE by comparing 1H-NMR, 13C- NMR, and MS data with literature values; these compounds were benzoic acid (1),[20] daphnoretin (2),[21] apigenin (3),[22] genkwanin (4),[23] taxifolin (5),[24] apigenin 7-O -glucoside (6),[25] luteolin 7-O -sambubioside (7),[26] genkwanin 5-O -glucoside (8),[27,28] matairesinoside (10),[29] and lariciresinol (11).[30]

Summary

Introduction

The current study was undertaken to isolate and identify anti-inflammatory and antiallergic active compounds in the ethyl acetate and butanol fractions of an ethanolic extract of the aerial parts of Stellera chamaejasme (SCAE). Natural Product Communications to inhibit IL-4 expression and β-hexosaminidase release in RBL-2 H3 mast cells (Figure 1). Ten other compounds were identified in SCAE by comparing 1H-NMR, 13C- NMR, and MS data with literature values; these compounds were benzoic acid (1),[20] daphnoretin (2),[21] apigenin (3),[22] genkwanin (4),[23] taxifolin (5),[24] apigenin 7-O -glucoside (6),[25] luteolin 7-O -sambubioside (7),[26] genkwanin 5-O -glucoside (8),[27,28] matairesinoside (10),[29] and lariciresinol (11).[30]

Results

Conclusion