Abstract
The exposure of reactive oxygen species is one of the aging triggers at cellular level. The antioxidants have been used as strategic efforts in overcoming the accumulation of ROS. Previous research using crude extracts of clove bud and leaves showed its potential as an antioxidant agent. However, no data were available regarding the antioxidant and antiaging activities of subsequent fractions of clove extracts. Therefore, this study aimed to analyze the antioxidant and antiaging activities of the n-hexane and ethanol fractions from clove bud and leaves. Antioxidant and antiaging activities were tested at the cellular level using the yeast model Schizosaccharomyces pombe. The highest flavonoid content was shown by clove leaf n-hexane fraction (25.6 mgQE·g−1). However, ethanol fraction of clove bud (FEB) showed the highest antioxidant activity based on TBA and antiglycation assays. FEB (8 μg·mL−1) and leaf ethanol fraction (FEL) (10 μg·mL−1) were able to induce yeast tolerance against oxidative stress. In addition, FEB could induce mitochondrial activity and delay the G1 phase of the cell cycle. FEB was found to be rich in gallic acid and (15Z)-9,12,13-trihydroxy-15-octadecenoic. Based on the data, FEB shows the potential antiaging activity, which is promising for further development as biopharmaceutical product formulations.
Highlights
Published: 7 October 2021The pharmaceutical industry has produced various antioxidant-based antiaging cosmetic and supplement products
Four sample fractions (FHB, fraction of clove bud (FEB), FHL, FEL) had antioxidant activity against DPPH radicals, which were significantly different from the positive control
Yeast grown in low glucose (0.3%) was designed as positive longevity control, while yeast grown in FEL: clove leaf ethanol fraction
Summary
The pharmaceutical industry has produced various antioxidant-based antiaging cosmetic and supplement products. The current trend in the cosmetics industry is an application of natural resources, including natural-based antioxidant compounds over synthetic ones as cosmetic ingredients. Such a strategy may potentially reduce the production cost and avoid adverse effects of the synthetic materials used [1]. Food and Drug Administration (US FDA) reported an increase in cases of adverse health effects on cosmetic products from 2015 as many as 706 to 1591 cases in 2016, and more than. Cellular aging can occur due to exposure and accumulation of reactive oxygen species (ROS) from UV lights, cigarette smoke, pollutants, and chemicals in cosmetic products. The exposure of ROS sources can accelerate cell aging, for example, in skin cells
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
