Anti-aging effect of black rice against H2O2-induced premature senescence

Abstract

This study was focused on the anti-aging effect of black rice under stress-induced premature senescence (SIPS) of WI-38 human diploid fibroblasts cells caused by hydrogen peroxide (H2O2), a well-established experimental model of cellular aging. The protective effect of the MeOH extract from black rice against H2O2-induced premature senescence was investigated using WI-38 cells by evaluating the cell viability, lipid peroxidation and cell life span. H2O2-treated WI-38 cells exhibited the phenomena of SIPS, the loss of cell viability, the increase of lipid peroxidation and shortening of the cell lifespan. However, the treatment with black rice attenuated cellular oxidative stress through increase in the cell viability and inhibition in lipid peroxidation. In addition, the life span of WI-38 cell showed extension of the population doubling level, suggesting that it would delay aging process. These results suggest that the MeOH extract from black rice may delay the aging process by attenuation of oxidative stress under SIPS cellular model. Key words: Black rice, ageing, WI-38 cell, premature senescence, oxidative stress.