Antiaging Activity of Peptide Identified from Fermented Trapa Japonica Fruit Extract in Human Dermal Fibroblasts.

Jin Dong Jang,Young-Joon Park,Kee-Young Lee,Young-Min Kim,Sang Moon Kang,Minkyung Kim,Gun-He Nam

doi:10.1155/2020/5895029

Abstract

We have previously shown that Trapa japonica fruit extract (TJE) as well as its fermented extract (FTJ) can be potentially used to treat alopecia. In the current study, a newly synthesized peptide (PEP) was detected in an active compound isolated from FTJ. Several biological assays were conducted to verify the antiaging effects of TJE, FTJ, and PEP on the skin. We examined the effects of TJE, FTJ, and PEP on cell viability, collagen synthesis, and inhibition of mRNA expression of matrix metalloproteinases (MMPs), induced by tumor necrosis factor alpha (TNF-α), in human dermal fibroblasts (HDFs). In addition, a wound-healing assay of the human keratinocyte cell line (HaCaT) and a clinical study of antiaging activity were conducted. The findings confirmed that PEP exerted an effect on cell proliferation in a dose-dependent manner. Treatment with TJE, FTJ, and PEP increased collagen synthesis but inhibited TNF-α-induced mRNA expression of MMPs. Compared with TJE and FTJ, PEP promoted a significant level of wound recovery in HaCaT cells and also exhibited antiaging effect, as demonstrated by a clinical study. These results suggest that PEP shows potential as a skin antiaging cosmetic product.

Highlights

Skin aging is a complex biological process involving both intrinsic and extrinsic factors [1]
Reactive oxygen species (ROS) cause skin aging by stimulating the expression of matrix metalloproteinases (MMPs) that contribute to skin aging by degrading various components of extracellular matrix (ECM) proteins such as collagen, fibronectin, elastin, and proteoglycans [7, 8]
The fermented biomass of T. japonica was extracted by using water as a solvent, and the extract was fractionated using silica gel and Sephadex LH-20 columns. e fraction with the highest protein content was selected, and further separation of the fraction was conducted through the RP silica column

Summary

Introduction

Skin aging is a complex biological process involving both intrinsic and extrinsic factors [1]. Intrinsic aging is due to inevitable, natural processes associated with genetics, hormones, and cellular metabolism, resulting in increased fine wrinkles and skin surface roughness as well as thin and dry skin. Extrinsic aging, which is characterized by coarse wrinkles, furrows, roughness, dryness, sagging skin, laxity, loss of elasticity, and pigmentation, is induced by long-term accumulation of external environmental factors such as sun exposure, pollution, smoking, and improper lifestyle [2, 3]. Focus on antiaging skin products has increased, with particular reference to the thriving cosmetics market [9]. Us, studies leading to the development of functional antiaging cosmetic products using natural ingredients, that are neither toxicants nor skin irritants, are actively being pursued [10] Focus on antiaging skin products has increased, with particular reference to the thriving cosmetics market [9]. us, studies leading to the development of functional antiaging cosmetic products using natural ingredients, that are neither toxicants nor skin irritants, are actively being pursued [10]

Methods

Results

Conclusion