Abstract
Uterine fibroids are a common type of solid tumor presenting in women of reproductive age. There are very few alternative treatment available from conventional treatment involving surgeries. Labisia pumila var. alata or locally known as 'Kacip Fatimah' was widely used as traditional medicine in Malaysia. This plant has been used to maintain a healthy female reproductive system. The present study aimed to evaluate anti fibroid potential of L. pumila extracts through in vitro apoptosis activity against uterine leiomyoma cells (SK-UT-1) and in uterine leiomyoma xenograft model. Evaluation of bioactive markers content were also carried out. Apoptotic induction of the extracts was determined by morphological examination of AO/PI dual staining assay by flourescent microscopy and flow cytometry analysis on Annexin V-FITC/PI stained cells. In vivo study was done in immune-compromised mouse xenograft model. HPLC analysis was employed to quantify marker compounds. Morphological analysis showed L. pumila induced apoptosis in a dose dependent manner against SK-UT-1 cells. In vivo study indicated that L. pumila significantly suppressed the growth of uterine fibroid tumor. All tested extracts contain bioactive marker of gallic acid and cafeic acid. This work provide significant data of the potential of L. pumila in management of uterine fibroids.<br />.
Highlights
Uterine fibroids, or leiomyomas, are the most common type of solid tumor presenting in women of reproductive age, with an incidence of 20-25% (Buttram and Reiter, 1981)
Determination of Marker Compounds by High Performance Liquid Chromatography (HPLC) analysis The identification of marker compounds in L. pumila extracts were determined by matching the retention time of the unknown peaks against standards eluted with same conditions as comparison
Quantification analysis showed the amount of Gallic acid (GA) and caffeic acid (CA) were varied within the extract types
Summary
Leiomyomas, are the most common type of solid tumor presenting in women of reproductive age, with an incidence of 20-25% (Buttram and Reiter, 1981). Accumulating evidences indicate that high intake of green vegetables and fruit seem to have a protective role and associated with reduced risk of uterine fibroids of US and Italian populations (Vidya and Nagini, 2012; Surh, 2003) This result supports the possible use of dietary phytochemicals for the prevention and treatment of uterine fibroids. The present study aimed to evaluate anti fibroid potential of L. pumila extracts through in vitro apoptosis activity against uterine leiomyoma cells (SK-UT-1) and in uterine leiomyoma xenograft model. Conclusion: This work provide significant data of the potential of L. pumila in management of uterine fibroids
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