Anti-ulcer effect of Ranunculus millefoliatus on absolute alcohol-induced stomach ulceration

Abstract

Ranunculus millefoliatus (RM) has been reported to have a numeral of biological properties. Though, the influence of this plant extract on stomach ulceration is yet stated. Thirty rats arbitrarily alienated 5 groups: the normal group, the ulcerated control group, the omeprazole group, and 2 investigational groups. Normal and ulcerated control groups were gavage by mouth 10% Tween 20. Omeprazole group fed orally 20 mg/kg omeprazole. Investigational group’s gavage of 250 mg/kg and 500 mg/kg ethanol extracted RM 10% Tween 20, correspondingly. Later another hour, the normal group gavage 10% Tween 20, and groups 2–5 gavage absolute ethanol. Afterward additional hours altogether rats were sacrificed. The ulcerated control group displayed extensive apparent stomach epithelial damage escorted by reduced stomachs mucus excretion and pH stomach contented. RM extract meaningfully condensed ethanol-induced gastric lacerations, for example, demonstrated via augmented gastric mucus and pH stomach contents, condensed ulceration expanse, decreased or lack of edema, and leucocyte penetration hypodermic coat. In stomach epithelial homogenate, RM extract revealed important upsurge superoxide dismutase (SOD), catalase (CAT) actions, expressively diminished malondialdehyde (MDA) level. Furthermore, RM extract augmented strength periodic acid–Schiff (PAS) stain stomach mucosa, besides formed up-regulation heat shock protein 70 (HSP 70) proteins down-regulation the Bcl-2-associated X protein (Bax) protein gastric mucosal. RM extract lessened the level of tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6), and improved the quantity of interleukin-10 (IL-10). Acute toxicity greater dosage of 500 mg/kg RM extract organized not obvious at all toxicology symbols might improve self-protective tools against stomach epithelial abrasions. RM extract presented gastroprotective effects that could be due to capability upsurge pH then mucus discharge, rise SOD and CAT, decrease MDA quantity, up-regulating HSP 70 proteins, down-regulating Bax protein, and moderate provocative cytokines.