Abstract
Objective To observe the anti-tumor effect and immune state in vivo induced by 4-1BBL-transfected prostate cancer RM-1 cells. Methods pcDNA3 and pcDNA3-4-1BBL were transferred into prostate cancer cells by LipofectamineTM 2000 respectively. The transfected cells were selected by G418.The expression of 4-1BBL was detected by reverse transcription-polymerase chain reaction (RT-PCR) and indirect immunofluorescent method (IF). The tumor size was observed after RM-1/4-1BBL cells, RM-1/pcD-NA3 cells and parental RM-1 cells were injected subcutaneously into C57BL/6 mice. The cytotoxicity of splenocytes from immunized mice was tested with CCK-8 method. Results 4-1BBL highly expressed RM-1 cells were constructed. On the 25th day, the tumor volume in RM-1/4-1BBL group (730. 0 ± 23. 6) mm3 was smaller than that in RM-1/pcDNA3 group[( 1490. 0 ±36. 4) mm3]and RM-1 group ( 1510. 0 ±26. 8) mm3(P＜0. 05). The CTL activity in RM-1/4-1BBL-immunized mice (35.4 ± 1.6)% was significantly higher than that in RM-1/pc1DNA3 group (12.8±2.0)% and RM-1 group (13.7 ±3.6)% (P＜0.05). Conclusion The prostate cancer cells transfected with 4-1BBL could significantly improve immune state and inhibit the tumor development. Key words: Prostate carcinoma; Tumor immunity
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