Abstract
In this study, we evaluated the effects of the extract of Huayu Jiedu (HYJD) formula on anti-tumor activity. It was found that HYJD treatment inhibited proliferation of seven cancer cell lines (IC50: 1.23-6.23 mg/mL). The gastric cancer cell line SGC-7901 was the most sensitive to HYJD. HYJD inhibited SGC-7901 cells proliferation in a dose- and time-dependant manner. More-over, HYJD significantly inhibited SGC-7901 cells adhesion, invasion and migration in a dose-dependent manner. qPCR results showed that HYJD treatment had no effect on PI3K, PDK1 and Akt mRNA expression. However, HYJD dramatically down-regulated Akt phosphorylation through inhibition of PDK1 kinase activity. Importantly, HYJD inhibited growth of tumor xenografts with no effect on body weight and spleen index. HYJD also significantly down-regulated Akt phosphorylation in tumor tissue. Taken together, this study demonstrated that HYJD exhibited an anti-tumor property, suggesting that HYJD may be a potential drug in the treatment of cancer.
Highlights
Gastric cancer is the fourth most common malignant tumor and the second leading cause of cancer-related mortality in all kinds of cancers worldwide (Jemal et al, 2011)
As a TCM formula, Huayu Jiedu (HYJD) consists of six Chinese medicines with percentages in weight as follows: Sculellaria barbata (50%), Costus root (16%), endothelium corneum gigeriae galli (16%), Pseudo-ginseng (8%), Gekko japonicus (5%), and scorpion (5%)
Various cancer cell lines including SGC-7901, A549, HepG2, Hela, Caco-2, MCF-7 and ECA-109 were incubated with different concentrations of HYJD for 48 hours. 50% inhibitory concentration (IC50 values) were determined by MTT assay
Summary
Gastric cancer is the fourth most common malignant tumor and the second leading cause of cancer-related mortality in all kinds of cancers worldwide (Jemal et al, 2011). Traditional Chinese Medicine has been applied in many fields of humanity health for thousands of years with its excellent achievements being increasingly acknowledged all over the world (Li et al, 2013). (HYJD) formula has been widely used for treatment of diseases such as hepatitis and rheumatoid arthritis with the action of supplementing the kidney, removing blood stasis and detoxicating (Cao et al, 2008). The recent study reported that HYJD formula in combination with chemotherapy could synergistically inhibit transplanted hepatocarcinoma in mice (Cao et al, 2005). As a TCM formula, HYJD consists of six Chinese medicines with percentages in weight as follows: Sculellaria barbata (50%), Costus root (16%), endothelium corneum gigeriae galli (16%), Pseudo-ginseng (8%), Gekko japonicus (5%), and scorpion (5%).
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